Recombinant human c-Mpl ligand is not a direct stimulator of proplatelet formation in mature human megakaryocytes

被引：40

作者：

Ito, T

Ishida, Y

Kashiwagi, R

Kuriya, S

机构：

[1] Division of Haematology, Third Dept. of Internal Medicine, Iwate Medical University, Iwate

[2] Division of Haematology, Third Dept. of Internal Medicine, Iwate Medical University, Iwate 020

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1996年 / 94卷 / 02期

关键词：

human bone marrow megakaryocytes; proplatelet formation; serum-free culture; c-Mpl ligand; interleukin-6;

D O I：

10.1046/j.1365-2141.1996.d01-1813.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To evaluate the effect of the c-Mpl ligand on platelet production by megakaryocytes, we investigated proplatelet formation in isolated human megakaryocytes cultured in serum-free medium, with or without the c-Mpl ligand, interleukin-6 and erythropoietin. When interleukin-6 was added to the culture medium, the percentage of megakaryocytes displaying proplatelets was approximately 1.5-fold the control value; whereas, in the presence of the c-Mpl ligand, the percentage of megakaryocytes displaying proplatelets decreased in a dose-dependent manner and did not increase compared to control values at any dose tested. However, the viability of megakaryocytes after a 4 d culture in the presence of the c-Mpl ligand was significantly higher than that of the cells cultured without it. The c-Mpl ligand did not stimulate the proplatelet formation in megakaryocytes in vitro.

引用

页码：387 / 390

页数：4

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