Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production

被引：129

作者：

Slater, S ^{[1
]}

Mitsky, TA ^{[1
]}

Houmiel, KL ^{[1
]}

Hao, M ^{[1
]}

Reiser, SE ^{[1
]}

Taylor, NB ^{[1
]}

Tran, M ^{[1
]}

Valentin, HE ^{[1
]}

Rodriguez, DJ ^{[1
]}

Stone, DA ^{[1
]}

Padgette, SR ^{[1
]}

Kishore, G ^{[1
]}

Gruys, KJ ^{[1
]}

机构：

[1] Monsanto Co, St Louis, MO 63198 USA

来源：

NATURE BIOTECHNOLOGY | 1999年 / 17卷 / 10期

关键词：

poly(hydroxyalkanoate); biodegradable polymer; green chemistry; PHA copolymer;

D O I：

10.1038/13711

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Poly(hydroxyalkanoates) are natural polymers with thermoplastic properties. One polymer of this class with commercial applicability, poly(3-hydroxybutyrate-co-3-hydroxyvale) (PHBV) can be produced by bacterial fermentation, but the process is not economically competitive with polymer production from petrochemicals, Poly(hydroxyalkanoate) production in green plants promises much lower costs, but producing copolymer with the appropriate monomer composition is problematic. In this study, we have engineered Arabidopsis and Brassica to produce PHBV in leaves and seeds, respectively, by redirecting the metabolic flow of intermediates from fatty acid and amino acid biosynthesis. We present a pathway for the biosynthesis of PHBV in plant plastids, and also report copolymer production, metabolic intermediate analyses, and pathway dynamics.

引用

页码：1011 / 1016

页数：6

共 34 条

[1] OCCURRENCE, METABOLISM, METABOLIC ROLE, AND INDUSTRIAL USES OF BACTERIAL POLYHYDROXYALKANOATES [J].