Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms

被引:85
作者
Hunt, L
Otterhag, L
Lee, JC
Lasheen, T
Hunt, J
Seki, M
Shinozaki, K
Sornmarin, M
Gilmour, DJ
Pical, C
Gray, JE [1 ]
机构
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England
[2] Lund Univ, Dept Plant Biochem, SE-22100 Lund, Sweden
[3] RIKEN Tsukuba Inst, Tsukuba, Ibaraki 3050074, Japan
[4] RIKEN Genom Sci Ctr, Tsurumi Ku, Kanagawa 2300045, Japan
关键词
Arabidopsis; calcium; gene expression; guard cell; phospholipase C; PI-PLC; pollen; stress;
D O I
10.1111/j.1469-8137.2004.01069.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
PI-PLCs synthesise the calcium releasing second messenger IP(3). We investigated the expression patterns of the Arabidopsis PI-PLC gene family and measured in vitro activity of encoded enzymes. Gene specific RT-PCR and promoter-GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli. All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1, and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1, 4 and 5 were induced by abiotic stresses. AtPLC1-5 were all shown to have phospholipase C activity in the presence of calcium ions. AtPLCs showed limited tissue specific expression and expression of at least three genes was increased by abiotic stress. The differing calcium sensitivities of recombinant AtPLC protein activities may provide a mechanism for generating calcium signatures.
引用
收藏
页码:643 / 654
页数:12
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