A Bistable Circuit Involving SCARECROW-RETINOBLASTOMA Integrates Cues to Inform Asymmetric Stem Cell Division

被引：274

作者：

Cruz-Ramirez, Alfredo ^{[2
]}

Diaz-Trivino, Sara ^{[2
]}

Blilou, Ikram ^{[2
]}

Grieneisen, Veronica A. ^{[1
,3
]}

Sozzani, Rosangela ^{[4
,5
]}

Zamioudis, Christos ^{[2
]}

Miskolczi, Pal ^{[6
,7
]}

Nieuwland, Jeroen ^{[8
]}

Benjamins, Rene ^{[2
]}

Dhonukshe, Pankaj ^{[2
]}

Caballero-Perez, Juan ^{[9
]}

Horvath, Beatrix ^{[2
]}

Long, Yuchen ^{[2
]}

Mahonen, Ari Pekka ^{[2
]}

Zhang, Hongtao ^{[2
]}

Xu, Jian ^{[2
]}

Murray, James A. H. ^{[8
]}

Benfey, Philip N. ^{[4
,5
]}

Bako, Laszlo ^{[6
,7
]}

Maree, Athanasius F. M. ^{[1
]}

Scheres, Ben ^{[2
]}

机构：

[1] John Innes Ctr, Dept Computat & Syst Biol, Norwich NR4 7UH, Norfolk, England

[2] Univ Utrecht, Dept Biol, NL-3584 CH Utrecht, Netherlands

[3] Univ E Anglia, Sch Biol Sci, Dept Cell & Dev Biol, Norwich NR4 7TJ, Norfolk, England

[4] Duke Univ, Dept Biol, Ctr Syst Biol, Durham, NC 27708 USA

[5] Duke Univ, Inst Genome Sci & Policy, Ctr Syst Biol, Durham, NC 27708 USA

[6] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden

[7] Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, Umea Plant Sci Ctr, S-90183 Umea, Sweden

[8] Cardiff Sch Biosci, Cardiff CF10 3AX, S Glam, Wales

[9] Inst Syst Biol, Seattle, WA 98109 USA

来源：

CELL | 2012年 / 150卷 / 05期

基金：

瑞典研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; ARABIDOPSIS ROOT-MERISTEM; TUMOR-SUPPRESSOR; SPLIT LUCIFERASE; AUXIN TRANSPORT; CYCLE; GENE; DIFFERENTIATION; PLANTS; GROWTH;

D O I：

10.1016/j.cell.2012.07.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plants, where cells cannot migrate, asymmetric cell divisions (ACDs) must be confined to the appropriate spatial context. We investigate tissue-generating asymmetric divisions in a stem cell daughter within the Arabidopsis root. Spatial restriction of these divisions requires physical binding of the stem cell regulator SCARECROW (SCR) by the RETINOBLASTOM-RELATED (RBR) protein. In the stem cell niche, SCR activity is counteracted by phosphorylation of RBR through a cyclinD6;1-CDK complex. This cyclin is itself under transcriptional control of SCR and its partner SHORT ROOT (SHR), creating a robust bistable circuit with either high or low SHR-SCR complex activity. Auxin biases this circuit by promoting CYCD6;1 transcription. Mathematical modeling shows that ACDs are only switched on after integration of radial and longitudinal information, determined by SHR and auxin distribution, respectively. Coupling of cell-cycle progression to protein degradation resets the circuit, resulting in a "flip flop" that constrains asymmetric cell division to the stem cell region.

引用

页码：1002 / 1015

页数：14

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