N-methyl-D-aspartate-induced long-term depression is associated with a decrease in postsynaptic protein kinase C substrate phosphorylation in rat hippocampal slices

被引：47

作者：

van Dam, EJM ^{[1
]}

Ruiter, B ^{[1
]}

Kamal, A ^{[1
]}

Ramakers, GMJ ^{[1
]}

Gispen, WH ^{[1
]}

de Graan, PNE ^{[1
]}

机构：

[1] Univ Utrecht, Med Ctr, Dept Pharmacol & Anat, Rudolf Magnus Inst Neurosci, NL-3508 AB Utrecht, Netherlands

来源：

NEUROSCIENCE LETTERS | 2002年 / 320卷 / 03期

关键词：

synaptic plasticity; growth-associated protein 43/B-50; RC3/neurogranin; hippocampus; protein phosphatase; phosphorylation;

D O I：

10.1016/S0304-3940(02)00037-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Incubation of rat hippocampal slices with a low concentration of N-methyl-D-aspartate (NMDA; 20 muM; 3 min) elicits a form of long-term depression (LTD). We used this chemical protocol to study the involvement of pre- and postsynaptic protein kinase/phosphatase activity in NMDA receptor-dependent LTD. We determined the phosphorylation states of a pre- and a postsynaptic protein kinase C substrate, B-50/growth-associated protein 43 (GAP43) and RC3, respectively, using quantitative immunoprecipitation. NMDA incubation resulted in a 2-amino-5-phosphonovalerate-sensitive long-lasting (>60 min) decrease in synaptic efficacy and a concomitant reduction in RC3 phosphorylation. B-50/GAP43 phosphorylation was unaffected. This suggests that NMDA-LTD, in contrast to low frequency-LTD, is only associated with activation of postsynaptic protein phosphatases. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：129 / 132

页数：4

共 22 条

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