Specific combinations of SR proteins associate with single pre-messenger RNAs in vivo and contribute different functions

被引：32

作者：

Bjork, Petra ^{[1
]}

Jin, ShaoBo ^{[1
]}

Zhao, Jian ^{[1
]}

Singh, Om Prakash ^{[1
]}

Persson, Jan-Olov ^{[2
]}

Hellman, Ulf ^{[3
]}

Wieslander, Lars ^{[1
]}

机构：

[1] Stockholm Univ, Dept Mol Biol & Funct Genom, SE-10691 Stockholm, Sweden

[2] Stockholm Univ, Dept Math, SE-10691 Stockholm, Sweden

[3] Ludwig Inst Canc Res, SE-75124 Uppsala, Sweden

来源：

JOURNAL OF CELL BIOLOGY | 2009年 / 184卷 / 04期

基金：

瑞典研究理事会;

关键词：

PRE-MESSENGER-RNA; SMALL NUCLEAR RIBONUCLEOPROTEIN; SPLICING SIGNAL INTERACTIONS; TRANSLATION INITIATION; SALIVARY-GLANDS; BALBIANI RINGS; FACTOR ASF/SF2; ALTERNATIVE; TRANSCRIPTION; CHIRONOMUS;

D O I：

10.1083/jcb.200806156

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Serine/arginine-rich (SR) proteins are required for messenger RNA (mRNA) processing, export, surveillance, and translation. We show that in Chironomus tentans, nascent transcripts associate with multiple types of SR proteins in specific combinations. Alternative splicing factor (ASF)/SF2, SC35, 9G8, and hrp45/SRp55 are all present in Balbiani ring (BR) pre-messenger ribonucleoproteins (mRNPs) preferentially when introns appear in the pre-mRNA and when cotranscriptional splicing takes place. However, hrp45/SRp55 is distributed differently in the pre-mRNPs along the gene compared with ASF/SF2, SC35, and 9G8, suggesting functional differences. All four SR proteins are associated with the BR mRNPs during export to the cytoplasm. Interference with SC35 indicates that SC35 is important for the coordination of splicing, transcription, and 3' end processing and also for nucleocytoplasmic export. ASF/SF2 is associated with polyribosomes, whereas SC35, 9G8, and hrp45/SRp55 cosediment with mono-ribosomes. Thus, individual endogenous pre-mRNPs/mRNPs bind multiple types of SR proteins during transcription, and these SR proteins accompany the mRNA and play different roles during the gene expression pathway in vivo.

引用

页码：555 / 568

页数：14

共 82 条

[1] Phosphorylation of serine 2 within the RNA polymerase IIC-terminal domain couples transcription and 3′ end processing [J].

Ahn, SH ;

Kim, M ;

Buratowski, S .

MOLECULAR CELL, 2004, 13 (01) :67-76

[2] Protein of the SR family of splicing factors binds extensively to exonic Balbiani ring pre-mRNA and accompanies the RNA from the gene to the nuclear pore [J].

AlzhanovaEricsson, AT ;

Sun, X ;

Visa, N ;

Kiseleva, E ;

Wurtz, T ;

Daneholt, B .

GENES & DEVELOPMENT, 1996, 10 (22) :2881-2893

[3] INDEPENDENT DEPOSITION OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS AND SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES AT SITES OF TRANSCRIPTION [J].

AMERO, SA ;

RAYCHAUDHURI, G ;

CASS, CL ;

VANVENROOIJ, WJ ;

HABETS, WJ ;

KRAINER, AR ;

BEYER, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (18) :8409-8413

[4] SPLICING OF BALBIANI RING-1 GENE PREMESSENGER RNA OCCURS SIMULTANEOUSLY WITH TRANSCRIPTION [J].

BAUREN, G ;

WIESLANDER, L .

CELL, 1994, 76 (01) :183-192

[5] Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3′-end formation and excision of the 3′-terminal intron [J].

Baurén, G ;

Belikov, S ;

Wieslander, L .

GENES & DEVELOPMENT, 1998, 12 (17) :2759-2769

[6] A nucleo-cytoplasmic SR protein functions in viral IRES-mediated translation initiation [J].

Bedard, Kristin M. ;

Daijogo, Sarah ;

Semler, Bert L. .

EMBO JOURNAL, 2007, 26 (02) :459-467

[7]

Björk P, 2006, MOL BIOL CELL, V17, P32, DOI 10.1091/mbc.E05-04-0339

[8] The Chironomus tentans translation initiation factor elF4H is present in the nucleus but does not bind to mRNA until the mRNA reaches the cytoplasmic perinuclear region [J].

Björk, P ;

Baurén, G ;

Gelius, B ;

Wrange, Ö ;

Wieslander, L .

JOURNAL OF CELL SCIENCE, 2003, 116 (22) :4521-4532

[9]

Bjork Petra, 2008, V464, P29

[10] A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm [J].

Cáceres, JF ;

Screaton, GR ;

Krainer, AR .

GENES & DEVELOPMENT, 1998, 12 (01) :55-66

← 1 2 3 4 5 6 7 8 9 →