The entire nucleotide sequence of a TT virus isolate from the united states (TUSO1): Comparison with reported isolates and phylogenetic analysis

被引:106
作者
Okamoto, H
Nishizawa, T
Ukita, M
Takahashi, M
Fukuda, M
Iizuka, H
Miyakawa, Y
Mayumi, M [1 ]
机构
[1] Jichi Med Sch, Div Immunol, Tochigi Ken 3290498, Japan
[2] Jichi Med Sch, Div Mol Virol, Tochigi Ken 3290498, Japan
[3] Inst Immunol, Tokyo 1120004, Japan
[4] Japanese Red Cross Saitama Blood Ctr, Saitama 3380001, Japan
[5] Miyakawa Mem Res Fdn, Tokyo 1070062, Japan
关键词
D O I
10.1006/viro.1999.9769
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A nonenveloped and single-stranded DNA virus designated TT virus (TTV) has been reported from Japan in association with hepatitis of unknown etiology. Very recently, the prototype TTV isolate (TA278) of genotype 1 is proven to have a circular genome with 3852 nucleotides. A TTV isolate (TUS01) was recovered from a blood donor in the United States, and its entire circular nucleotide sequence of 3818 nucleotides was determined. It possessed two open reading frames coding for 761 and 156 amino acids, respectively. TUS01 shared 60.5% of the nucleotide sequence with the TA278 isolate from Japan that was longer by 35 nt. The sequence of the noncoding region of 1203 nt was conserved with a similarity of 83.4%. Sequence preservation was much lower for the two open reading frames; nucleotide and amino acid sequences were 54.8 and 37.0% similar, respectively, for one and 55.5 and 38.8% similar for the other. By comparison of a partial sequence of 222 nucleotides among 239 TTV isolates available from various countries, at least 11 genotypes with sequence divergence of >30% were recognized. TUS01 was deduced to be of genotype 11, which has not been reported before. Conserved sequences in the noncoding region could be used as primers for sensitively detecting TTV DNA by polymerase chain reaction. Divergent sequences in coding regions would be useful as primers for distinguishing various TTV genotypes. (C) 1999 Academic Press.
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页码:437 / 448
页数:12
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