Induced heteroduplex genotyping of TNF-α, IL-1β, IL-6 and IL-10 polymorphisms associated with transcriptional regulation

We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-alpha, IL-1 beta, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-alpha positions - 308 and - 238; IL-1 beta position +3953; IL-6 position - 174; and IL-10 positions - 1082, - 819 and - 592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors. (C) 1999 Academic Press.