Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment

被引:166
作者
Bak, Jin Seop [1 ]
Ko, Ja Kyong [1 ]
Han, Young Hwan [3 ]
Lee, Byung Cheol [3 ]
Choi, In-Geol [2 ]
Kim, Kyoung Heon [1 ,2 ]
机构
[1] Korea Univ, Brain Korea Sch Life Sci & Biotechnol 21, Seoul 136713, South Korea
[2] Korea Univ, Coll Life Sci & Biotechnol, Seoul 136713, South Korea
[3] Korea Atom Energy Res Inst, Quantum Opt Res Div, Taejon 305353, South Korea
关键词
Electron beam irradiation; Lignocellulose; Biomass; Pretreatment; Biofuel; DILUTE-ACID HYDROLYSIS; CORN STOVER; CELLULOSE; ETHANOL; BIOMASS; OPTIMIZATION; MECHANISM;
D O I
10.1016/j.biortech.2008.09.010
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Rice straw was irradiated using an electron beam at currents and then hydrolyzed with cellulase and beta-glucosidase to produce glucose. The pretreatment by electron beam irradiation (EBI) was found to significantly increase the enzyme digestibility of rice straw. Specifically, when rice straw that was pretreated by EBI at 80 kGy at 0.12 mA and 1 MeV was hydrolyzed with 60 FPU of cellulase and 30 CBU of beta-glucosidase, the glucose yield after 132 h of hydrolysis was 52.1% of theoretical maximum. This value was significantly higher than the 22.6% that was obtained when untreated rice straw was used. In addition, SEM analysis of pretreated rice straw revealed that EBI caused apparent damage to the surface of the rice straw. Furthermore, EBI pretreatment was found to increase the crystalline portion of the rice straw. Finally, the crystallinity and enzyme digestibility were found to be strongly correlated between rice straw samples that were pretreated by EBI under different conditions. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1285 / 1290
页数:6
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