Molecular weight and aggregation behaviour in solution of beta-D-glucan from Poria cocos sclerotium.

被引：43

作者：

Zhang, L ^{[1
]}

Ding, Q ^{[1
]}

Zhang, PY ^{[1
]}

Zhu, RP ^{[1
]}

Zhou, YH ^{[1
]}

机构：

[1] NATL RES CTR CERTIFIED REFERENCE MAT,BEIJING 100013,PEOPLES R CHINA

来源：

CARBOHYDRATE RESEARCH | 1997年 / 303卷 / 02期

关键词：

Poria cocos; polysaccharide; molecular weight; light scattering; intrinsic viscosity; aggregation;

D O I：

10.1016/S0008-6215(97)00172-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The weight-average molecular weights M-W and intrinsic viscosities [eta] of Linear beta-(1 --> 3) -D-glucan (PC3), a major polysaccharide in Poria cocos sclerotium were studied by light scattering and viscometry. The M-W values of glucan PC3 fractions in 20% cadoxen solution and in cadoxen were 20.6 X 10(4) and 8.93 X 10(4), respectively. The [eta] values of glucan PC3 in water-cadoxen mixtures underwent an almost discontinuous decrease in the range from 20 to 30% cadoxen. The data (M-W, [eta]) of the unfractionated PC3 and of two fractions in 20% cadoxen and in cadoxen showed that glucan PC3 forms aggregates in 20% cadoxen solution, and dissociates to single chains in cadoxen. The change of aggregation and disaggregation in water-cadoxen mixtures are reversible. (C) 1997 Elsevier Science Ltd.

引用

页码：193 / 197

页数：5

共 15 条

[1] ANTITUMOUR POLYSACCHARIDE DERIVED CHEMICALLY FROM NATURAL GLUCAN (PACHYMAN) [J].

CHIHARA, G ;

HAMURO, J ;

MAEDA, Y ;

ARAI, Y ;

FUKUOKA, F .

NATURE, 1970, 225 (5236) :943-+

[2] CURDLAN - A BACTERIAL GEL-FORMING BETA-1 3-GLUCAN [J].

HARADA, T ;

MISAKI, A ;

SAITO, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 124 (1-3) :292-&

[3] STRUCTURE AND MOLECULAR SIZE OF PACHYMAN [J].

HOFFMANN, GC ;

SIMSON, BW ;

TIMELL, TE .

CARBOHYDRATE RESEARCH, 1971, 20 (01) :185-&

[4] UNPERTURBED DIMENSIONS OF AMYLOSE IN BINARY WATER-DIMETHYL SULFOXIDE MIXTURES [J].

JORDAN, RC ;

BRANT, DA .

MACROMOLECULES, 1980, 13 (03) :491-499

[5] STUDIES ON THE ANTITUMOR ACTIVE POLYSACCHARIDES FROM THE MYCELIA OF PORIA-COCOS WOLF .3. ANTITUMOR-ACTIVITY AGAINST MOUSE-TUMORS [J].

KANAYAMA, H ;

TOGAMI, M ;

ADACHI, N ;

FUKAI, Y ;

OKUMOTO, T .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1986, 106 (04) :307-312

[6] STUDIES ON THE ANTITUMOR ACTIVE POLYSACCHARIDES FROM THE MYCELIA OF PORIA-COCOS WOLF .2. STRUCTURAL-ANALYSIS OF ANTITUMOR POLYSACCHARIDE H-11 [J].

KANAYAMA, H ;

ADACHI, N ;

FUKAI, Y ;

TAKEUCHI, I ;

TOGAMI, M .

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 1986, 106 (03) :206-211

[7] FINE-STRUCTURE OF (1-]3)-BETA-D-GLUCANS - CURDLAN AND PARAMYLON [J].

MARCHESSAULT, RH ;

DESLANDES, Y .

CARBOHYDRATE RESEARCH, 1979, 75 (OCT) :231-242

[8] A POLYSACCHARIDE PRODUCED BY LABORATORY CULTIVATION OF PORIA-COCOS WOLF [J].

NARUI, T ;

TAKAHASHI, K ;

KOBAYASHI, M ;

SHIBATA, S .

CARBOHYDRATE RESEARCH, 1980, 87 (01) :161-163

[9] TRIPLE HELIX OF A SCHIZOPHYLLUM-COMMUNE POLYSACCHARIDE IN AQUEOUS-SOLUTION [J].

NORISUYE, T ;

YANAKI, T ;

FUJITA, H .

JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 1980, 18 (03) :547-558

[10] A COMPARARISON OF STRUCTURE OF CURDLAN PACHYMAN [J].

SAITO, H ;

MISAKI, A ;

HARADA, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1968, 32 (10) :1261-&

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