ANTI-OXIDANT, ANTI-DIABETIC, AND ANTI-HYPERTENSIVE EFFECTS OF EXTRACTED PHENOLICS AND HYDROLYZED PEPTIDES FROM BARLEY PROTEIN FRACTIONS

被引:52
作者
Alu'datt, Muhammad H. [1 ]
Ereifej, Khalil [1 ]
Abu-Zaiton, Ahmed [2 ]
Alrababah, Mohammad [3 ]
Almajwal, Ali [4 ]
Rababah, Taha [1 ]
Yang, Wade [5 ]
机构
[1] Jordan Univ Sci & Technol, Dept Nutr & Food Technol, Irbid, Jordan
[2] Al Albayt Univ, Dept Biol Sci, Mafraq, Jordan
[3] Jordan Univ Sci & Technol, Dept Nat Resources & Environm, Irbid, Jordan
[4] King Saud Univ, Coll Appl Med Sci, Dept Community Hlth Sci, Riyadh, Saudi Arabia
[5] Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA
关键词
Phenolic compounds; Peptide; alpha-Amylase inhibitor; Angiotensin I-converting enzyme inhibitor; Antioxidant activity; Globulin; Glutein-1; Glutein-2; Prolamin; Protein isolate; Barley flour; ENZYME INHIBITORY PEPTIDE; ANGIOTENSIN-CONVERTING-ENZYME; SPECTROPHOTOMETRIC ASSAY; PURIFICATION; CAPACITY; AMYLASE; FLOUR; FOODS;
D O I
10.1080/10942912.2010.503357
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
This study was conducted to search for novel natural bioactive compounds (peptides and phenolic compounds) with hypoglycemic, antioxidant, and anti-diabetic potential from barley protein fractions and isolates. Protein isolate and globulin, prolamin, glutein-1, and glutein-2 fractions of barley flour were extracted from barley flour. Free and bound phenolic compounds were extracted from protein isolate and protein fractions. Protein isolate and protein fractions from barley were subjected to pancreatic hydrolysis to obtain peptides. Peptide and both free and bound phenolic compounds were subjected to determine antioxidant and their potential to inhibit the key enzymes relevant to diabetes and hypertension using in vitro models. The extracted phenolic from prolamin fraction and protein isolate revealed the highest content of total phenolics (2.0-2.4 mg/g), antioxidant activity (65-73%), angiotensin converting enzyme-inhibitory activity (73-87%), and alpha-amylase inhibitory activity (57-77%) as compared to that of other protein fractions. Hydrolysates of prolamin fraction exhibited the highest antioxidant activity (77.7%) and angiotensin converting enzyme-inhibitory activity (61.3%) as compared to all other protein fractions and protein isolate. Poor correlations were obtained between total phenolic content, antioxididant activity, ACE-inhibitory activity, and alpha-amylase inhibitory activity of phenolic compounds from protein fractions and isolate. On the other hand, positive correlations were obtained between antioxididant activities, angiotensin converting enzyme-inhibitory activity, and the degree of hydrolysis of peptides from hydrolyzed protein fractions and protein isolate. Our findings indicated that prolamin fraction and protein isolate were recommended to be used as hypoglycemic, antioxidant, and anti-diabetic ingredients as potential candidates in functional, nutraceutical, and pharmaceutical industries.
引用
收藏
页码:781 / 795
页数:15
相关论文
共 42 条
[1]
α-amylase inhibitory activity of some Malaysian plants used to treat diabetes;: with particular reference to Phyllanthus amarus [J].
Ali, Hasenah ;
Houghton, P. J. ;
Soumyanath, Amala .
JOURNAL OF ETHNOPHARMACOLOGY, 2006, 107 (03) :449-455
[2]
[Anonymous], 1994, Antioxidants in Nutrition, Health and Disease
[3]
CARBOHYDRATE AND PHENOLIC CONSTITUENTS IN A COMPREHENSIVE RANGE OF RAPESEED AND CANOLA FRACTIONS - NUTRITIONAL SIGNIFICANCE FOR ANIMALS [J].
BLAIR, R ;
REICHERT, RD .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1984, 35 (01) :29-35
[4]
High (1→3,1→4)-β-glucan barley fractions in bread making and their effects on human glycemic response [J].
Cavallero, A ;
Empilli, S ;
Brighenti, F ;
Stanca, AM .
JOURNAL OF CEREAL SCIENCE, 2002, 36 (01) :59-66
[5]
STRUCTURAL-ANALYSIS OF ANTIOXIDATIVE PEPTIDES FROM SOYBEAN BETA-CONGLYCININ [J].
CHEN, HM ;
MURAMOTO, K ;
YAMAUCHI, F .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (03) :574-578
[6]
Clonal variation in raspberry fruit phenolics and relevance for diabetes and hypertension management [J].
Cheplick, Susan ;
Kwon, Young-In ;
Bhowmik, Prasanta ;
Shetty, Kalidas .
JOURNAL OF FOOD BIOCHEMISTRY, 2007, 31 (05) :656-679
[7]
SPECTROPHOTOMETRIC ASSAY USING ORTHO-PHTHALDIALDEHYDE FOR DETERMINATION OF PROTEOLYSIS IN MILK AND ISOLATED MILK-PROTEINS [J].
CHURCH, FC ;
SWAISGOOD, HE ;
PORTER, DH ;
CATIGNANI, GL .
JOURNAL OF DAIRY SCIENCE, 1983, 66 (06) :1219-1227
[8]
SPECTROPHOTOMETRIC ASSAY AND PROPERTIES OF ANGIOTENSIN-CONVERTING ENZYME OF RABBIT LUNG [J].
CUSHMAN, DW ;
CHEUNG, HS .
BIOCHEMICAL PHARMACOLOGY, 1971, 20 (07) :1637-+
[9]
Antioxidant capacity of oat (Avena sativa L.) extracts.: 2.: In vitro antioxidant activity and contents of phenolic and tocol antioxidants [J].
Emmons, CL ;
Peterson, DM ;
Paul, GL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (12) :4894-4898
[10]
Effect of barley flour on quality of Balady bread [J].
Ereifej, KI ;
Al-Mahasneh, MA ;
Rababah, TM .
INTERNATIONAL JOURNAL OF FOOD PROPERTIES, 2006, 9 (01) :39-49