Canonical and atypical E2Fs regulate the mammalian endocycle

被引：126

作者：

Chen, Hui-Zi ^{[1
,2
,3
]}

Ouseph, Madhu M. ^{[1
,4
]}

Li, Jing ^{[1
]}

Pecot, Thierry ^{[1
]}

Chokshi, Veda ^{[1
]}

Kent, Lindsey ^{[1
]}

Bae, Sooin ^{[1
]}

Byrne, Morgan ^{[1
]}

Duran, Camille ^{[1
]}

Comstock, Grant ^{[1
]}

Trikha, Prashant ^{[1
]}

Mair, Markus ^{[1
]}

Senapati, Shantibhusan ^{[1
]}

Martin, Chelsea K. ^{[1
]}

Gandhi, Sagar ^{[1
]}

Wilson, Nicholas ^{[1
]}

Liu, Bin ^{[1
]}

Huang, Yi-Wen ^{[5
]}

Thompson, John C. ^{[1
]}

Raman, Sundaresan

Singh, Shantanu

Leone, Marcelo

Machiraju, Raghu

Huang, Kun

Mo, Xiaokui ^{[6
]}

Fernandez, Soledad ^{[6
]}

Kalaszczynska, Ilona ^{[7
,8
]}

Wolgemuth, Debra J. ^{[9
,10
]}

Sicinski, Piotr ^{[7
,8
]}

Huang, Tim ^{[1
]}

Jin, Victor

Leone, Gustavo ^{[1
,2
,3
]}

机构：

[1] Ohio State Univ, Dept Mol Genet, Dept Mol Virol Immunol & Med Genet, Solid Tumor Biol Program,Comprehens Canc Ctr, Columbus, OH 43210 USA

[2] Ohio State Univ, Coll Med, Med Scientist Training Program, Columbus, OH 43210 USA

[3] Ohio State Univ, Coll Med, Integrated Biomed Grad Program, Columbus, OH 43210 USA

[4] Ohio State Univ, Ohio State Biochem Program, Columbus, OH 43210 USA

[5] Med Coll Wisconsin, Dept Obstet & Gynecol, Milwaukee, WI 53226 USA

[6] Ohio State Univ, Ctr Biostat, Off Hlth Sci, Columbus, OH 43210 USA

[7] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02215 USA

[8] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02215 USA

[9] Columbia Univ, Med Ctr, Dept Genet & Dev, Dept Obstet & Gynecol,Inst Human Nutr, New York, NY 10032 USA

[10] Columbia Univ, Med Ctr, Herbert Irving Comprehens Canc Ctr, New York, NY 10032 USA

来源：

NATURE CELL BIOLOGY | 2012年 / 14卷 / 11期

关键词：

S-PHASE; LIVER; MICE; ENDOREPLICATION; CELLS; POLYPLOIDY; REPRESSION; ACTIVATORS; PATTERNS; GROWTH;

D O I：

10.1038/ncb2595

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The endocycle is a variant cell cycle consisting of successive DNA synthesis and gap phases that yield highly polyploid cells. Although essential formetazoan development, relatively little is known about its control or physiologic role in mammals. Using lineage-specific cre mice we identified two opposing arms of the E2F program, one driven by canonical transcription activation (E2F1, E2F2 and E2F3) and the other by atypical repression (E2F7 and E2F8), that converge on the regulation of endocycles in vivo. Ablation of canonical activators in the two endocycling tissues of mammals, trophoblast giant cells in the placenta and hepatocytes in the liver, augmented genome ploidy, whereas ablation of atypical repressors diminished ploidy. These two antagonistic arms coordinate the expression of a unique G2/M transcriptional program that is critical for mitosis, karyokinesis and cytokinesis. These results provide in vivo evidence for a direct role of E2F family members in regulating non-traditional cell cycles in mammals.

引用

页码：1192 / +

页数：22

共 60 条

[1] CORRELATION BETWEEN TISSULAR AND DIVISION FUNCTIONS IN LIVER OF YOUNG RATS [J].

BARBASON, H ;

VANCANTF.J ;

HOUBRECHTS, N .

CELL AND TISSUE KINETICS, 1974, 7 (04) :319-326

[2] A comparison of normalization methods for high density oligonucleotide array data based on variance and bias [J].

Bolstad, BM ;

Irizarry, RA ;

Åstrand, M ;

Speed, TP .

BIOINFORMATICS, 2003, 19 (02) :185-193

[3] Developmental control of endocycles and cell growth in plants [J].

Breuer, Christian ;

Ishida, Takashi ;

Sugimoto, Keiko .

CURRENT OPINION IN PLANT BIOLOGY, 2010, 13 (06) :654-660

[4] Emerging roles of E2Fs in cancer: an exit from cell cycle control [J].

Chen, Hui-Zi ;

Tsai, Shih-Yin ;

Leone, Gustavo .

NATURE REVIEWS CANCER, 2009, 9 (11) :785-797

[5] E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells [J].

Chong, Jean-Leon ;

Wenzel, Pamela L. ;

Saenz-Robles, M. Teresa ;

Nair, Vivek ;

Ferrey, Antoney ;

Hagan, John P. ;

Gomez, Yorman M. ;

Sharma, Nidhi ;

Chen, Hui-Zi ;

Ouseph, Madhu ;

Wang, Shu-Huei ;

Trikha, Prashant ;

Culp, Brian ;

Mezache, Louise ;

Winton, Douglas J. ;

Sansom, Owen J. ;

Chen, Danian ;

Bremner, Rod ;

Cantalupo, Paul G. ;

Robinson, Michael L. ;

Pipas, James M. ;

Leone, Gustavo .

NATURE, 2009, 462 (7275) :930-934

[6] Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription [J].

Christensen, J ;

Cloos, P ;

Toftegaard, U ;

Klinkenberg, D ;

Bracken, AP ;

Trinh, E ;

Heeran, M ;

Di Stefano, L ;

Helin, K .

NUCLEIC ACIDS RESEARCH, 2005, 33 (17) :5458-5470

[7] IFPA 2004 Award In Placentology Lecture - How to make a placenta: Mechanisms of trophoblast cell differentiation in mice - A review [J].

Cross, JC .

PLACENTA, 2005, 26 :S3-S9

[8] DIURNAL PATTERNS OF DNA-SYNTHESIS IN RAT - MODIFICATION BY DIET AND FEEDING SCHEDULE [J].

DALLMAN, PR ;

SPIRITO, RA ;

SIIMES, MA .

JOURNAL OF NUTRITION, 1974, 104 (10) :1234-1241

[9] Identification and characterization of E2F7, a novel mammalian E2F family member capable of blocking cellular proliferation [J].

de Bruin, A ;

Maiti, B ;

Jakoi, L ;

Timmers, C ;

Buerki, R ;

Leone, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) :42041-42049

[10] E2F7, a novel E2F featuring DP-independent repression of a subset of E2F-regulated genes [J].

Di Stefano, L ;

Jensen, MR ;

Helin, K .

EMBO JOURNAL, 2003, 22 (23) :6289-6298

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