A polysaccharide from Armillaria mellea exhibits strong in vitro anticancer activity via apoptosis-involved mechanisms

被引：122

作者：

Wu, Jun ^{[1
]}

Zhou, Jinxu ^{[2
]}

Lang, Yaoguo ^{[1
]}

Yao, Lei ^{[1
]}

Xu, Hai ^{[1
]}

Shi, Hubo ^{[1
]}

Xu, Shidong ^{[1
]}

机构：

[1] Harbin Med Univ, Affiliated Tumor Hosp 3, Dept Thorac Surg, Harbin 150040, Peoples R China

[2] Harbin Inst Technol, Dept Foreign Languages, Harbin 150001, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 51卷 / 04期

关键词：

Armillaria mellea; Polysaccharide; Apoptosis; Caspase; Mitochondrial membrane potential; CANCER CELLS; SUBSTANCES; MUSHROOMS;

D O I：

10.1016/j.ijbiomac.2012.06.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Armillaria mellea is a famous traditional Chinese medicinal and edible fungus. In this study, we purified a water-soluble polysaccharide (AMP) from the fruiting bodies of this fungus. AMP contained 94.8% carbohydrate, 2.3% uronic acid and 0.5% protein. Its molecular weight was determined as 4.6 x 10(5) Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis indicated that AMP was mainly composed of o-glucose. In vitro assay. AMP exhibited a potent tumor growth inhibitory effect on A549 cells, and induced cell cycle disruption in the GO/G1 phase, accompanied by an increment of apoptotic cells. Furthermore, AMP induced the disruption of mitochondrial membrane potential, thus leading to cytochrome c release from mitochondria and activation of caspase-3 and -9. Taken together, our results demonstrate that AMP possesses strong antitumor activities through the mitochondria dependent pathway and activation of caspase cascade through cytochrome c release. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：663 / 667

页数：5

共 24 条

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