Biological response of human bone marrow stromal cells to sandblasted titanium nitride-coated implant surfaces

被引：34

作者：

Annunziata, Marco ^{[1
]}

Guida, Luigi ^{[1
]}

Perillo, Letizia ^{[1
]}

Aversa, Raffaella ^{[2
]}

Passaro, Irene ^{[3
]}

Oliva, Adriana ^{[3
]}

机构：

[1] Univ Naples 2, Dept Odontostomatol Orthodont & Surg Disciplines, I-80138 Naples, Italy

[2] Univ Naples Federico 2, Sch Engn, Dept Mat & Prod Engn DIMP, I-80125 Naples, Italy

[3] Univ Naples 2, Dept Biochem & Biophys F Cedrangolo, I-80138 Naples, Italy

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2008年 / 19卷 / 12期

关键词：

D O I：

10.1007/s10856-008-3514-2

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

Titanium nitride (TiN) coating has been proposed as an adjunctive surface treatment aimed to increase the physico-mechanical and aesthetic properties of dental implants. In this study we investigated the biological response of primary human bone marrow stromal cells (BMSC) to TiN-coated sandblasted (TiN-SB) compared to uncoated sandblasted (SB) surfaces. SB and TiN-SB disks were qualitatively and quantitatively analyzed by atomic force microscopy. BMSC were obtained from healthy donors and their adhesion and proliferation on the titanium disks were evaluated by scanning electron microscopy and viability assay. The osteoblastic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis, and extracellular mineralization, was assessed by specific immunoenzymatic or spectrophotometric assays. No difference (P > 0.05) between TiN-SB and SB disks was found in terms of any of the investigated parameters. TiN-coating showed to maintain the topographical characteristics of sandblasted titanium surfaces and their biological affinity toward bone precursors.

引用

页码：3585 / 3591

页数：7

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