Cell fusion in osteoclasts plays a critical role in controlling bone mass and osteoblastic activity

被引：52

作者：

Iwasaki, Ryotaro ^{[1
,4
]}

Ninomiya, Ken ^{[1
,2
]}

Miyamoto, Kana ^{[1
,2
]}

Suzuki, Toru ^{[1
,2
]}

Sato, Yuiko ^{[1
,2
,3
]}

Kawana, Hiromasa ^{[4
]}

Nakagawa, Taneaki ^{[4
]}

Suda, Toshio ^{[1
]}

Miyamoto, Takeshi ^{[1
,2
,3
,5
]}

机构：

[1] Keio Univ, Sch Med, Sakaguchi Lab Dev Biol, Dept Cell Differentiat,Shinjuku Ku, Tokyo 1608582, Japan

[2] Keio Univ, Sch Med, Dept Orthoped Surg, Shinjuku Ku, Tokyo 1608582, Japan

[3] Keio Univ, Sch Med, Dept Musculoskeletal Reconstruct & Regenerat Surg, Shinjuku Ku, Tokyo 1608582, Japan

[4] Keio Univ, Sch Med, Dept Dent & Oral Surg, Shinjuku Ku, Tokyo 1608582, Japan

[5] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol, Kawaguchi, Saitama 3320012, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 03期

基金：

日本学术振兴会;

关键词：

DC-STAMP; Osteoclast; Bone homeostasis; Transgenic; Cell-cell fusion; Uncoupling;

D O I：

10.1016/j.bbrc.2008.10.076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The balance between osteoclast and osteoblast activity is central for maintaining the integrity of bone homeostasis. Here we show that mice lacking dendritic cell specific transmembrane protein (DC-STAMP), an essential molecule for osteoclast cell-cell fusion, exhibited impaired bone resorption and upregulation of bone formation by osteoblasts, which do not express DC-STAMP, which led to increased bone mass. On the contrary, DC-STAMP over-expressing transgenic (DC-STAMP-Tg) mice under the control of an actin promoter showed significantly accelerated cell-cell fusion of osteoclasts and bone resorption, with decreased osteoblastic activity and bone mass. Bone resorption and formation are known to be regulated in a coupled manner, whereas DC-STAMP regulates bone homeostasis in an un-coupled manner. Thus our results indicate that inhibition of a single molecule provides both decreased osteoclast activity and increased bone formation by osteoblasts, thereby increasing bone mass in an un-coupled and a tissue specific manner. (C) 2008 Elsevier Inc. All rights reserved

引用

页码：899 / 904

页数：6

共 21 条

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osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification [J].