Recognition and repair of UV lesions in loop structures of duplex DNA by DASH-type cryptochrome

被引：143

作者：

Pokorny, Richard ^{[1
]}

Klar, Tobias ^{[2
]}

Hennecke, Ulrich ^{[3
]}

Carell, Thomas ^{[3
]}

Batschauer, Alfred ^{[1
]}

Essen, Lars-Oliver ^{[2
]}

机构：

[1] Univ Marburg, Dept Biol, D-35032 Marburg, Germany

[2] Univ Marburg, Dept Chem, D-35032 Marburg, Germany

[3] Univ Munich, Ctr Integrat Prot Sci, Dept Chem & Biochem, D-81377 Munich, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 52期

关键词：

Arabidopsis; DNA repair; photolyase;

D O I：

10.1073/pnas.0805830106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA photolyases and cryptochromes (cry) form a family of flavoproteins that use light energy in the blue/UV-A region for the repair of UV-induced DNA lesions or for signaling, respectively. Very recently, it was shown that members of the DASH cryptochrome subclade repair specifically cyclobutane pyrimidine dimers (CPDs) in UV-damaged single-stranded DNA. Here, we report the crystal structure of Arabidopsis cryptochrome 3 with an in-situ-repaired CPD substrate in single-stranded DNA. The structure shows a binding mode similar to that of conventional DNA photolyases. Furthermore, CPD lesions in double-stranded DNA are bound and repaired with similar efficiency as in single-stranded DNA if the CPD lesion is present in a loop structure. Together, these data reveal that DASH cryptochromes catalyze light-driven DNA repair like conventional photolyases but lack an efficient flipping mechanism for interaction with CPD lesions within duplex DNA.

引用

页码：21023 / 21027

页数：5

共 35 条

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