Putative P-glycoprotein expression in arsenite-resistant Leishmania donovani down-regulated by verapamil

被引：25

作者：

Kaur, J ^{[1
]}

Dey, CS ^{[1
]}

机构：

[1] NIPER, Dept Biotechnol, Punjab 160062, India

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 271卷 / 03期

关键词：

arsenite resistance; P-glycoprotein; verapamil; L; donovani;

D O I：

10.1006/bbrc.2000.2680

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Western immunoblots of whole cell lysate and crude membrane extract of an in vitro selected sodium m-arsenite-resistant L. donovani strain revealed a 230-kDa protein identified by an anti-P-glycoprotein (Pgp) antibody. Immunofluorescence microscopy, using the same antibody, detected putative Pgp on resistant parasites. Overexpression of the putative Pgp was downregulated by verapamil. These results provided, possibly, the first evidence that (i) overexpression of Pgp-like protein occurs in arsenite-resistant Leishmania that are cross-resistant to structurally and functionally unrelated drugs and (ii) verapamil regulates drug sensitivity possibly by down-regulating Pgp expression in the arsenite-resistant Leishmania. (C) 2000 Academic Press.

引用

页码：615 / 619

页数：5

共 29 条

[1] PARTIAL-PURIFICATION AND RECONSTITUTION OF THE HUMAN MULTIDRUG-RESISTANCE PUMP - CHARACTERIZATION OF THE DRUG-STIMULATABLE ATP HYDROLYSIS [J].