Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates

被引：147

作者：

Su, J ^{[1
]}

Mrksich, M ^{[1
]}

机构：

[1] Univ Chicago, Dept Chem, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2002年 / 41卷 / 24期

关键词：

D O I：

10.1002/anie.200290026

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peptide and carbohydrate biochips can be assayed with MALDI mass spectrometry. Self-assembled monolayers (SAMs) that are modified with ligands provide a platform for identifying selective protein-ligand and enzyme-substrate interactions. MALDI provides a rapid and sensitive method for detection and avoids the need to label protein analytes with fluorescent or radioactive tags. The picture shows the mass spectrum of a SAM presenting a carbohydrate.

引用

页码：4715 / 4718

页数：4

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