A derivatizing reagent-kit using a single coumarin fluorophore

6,7-Dimethoxycoumarins having various reactive groups such as bromoacetyl-, hydrazinocarbonyl- and chlorocarbonyl-groups at 3-position on coumarin ring were synthesized for the construction of a fluorescent derivatizing reagent-kit for carboxylic acids, aldehyde/ketone, alcohol, phenol and amines. These reagents were evaluated from their reactivities with model compounds and fluorescent characteristics of products obtained. Of the reagents, 3-bromoacetyl- and 3-chlorocarbonyl-6,7-dimethoxycoumarins were found to afford highly fluorescent products with above 0.8 in quantum yields for carboxylic acids, alcohol and aliphatic amines. It was found that the construction of reagent-kit by using a single fluorophore selected after consideration of its emission mechanism can be significant and useful as an approach for the efficient reagent development.