Crystallization and X-ray crystallographic analysis of m-calpain, a Ca2+-dependent protease

被引：12

作者：

Hosfield, CM

Ye, QL

Arthur, JSC

Hegadorn, C

Croall, DE

Elce, JS

Jia, ZC ^{[1
]}

机构：

[1] Queens Univ, Prot Engn Network Ctr Excellence, Kingston, ON K7L 3N6, Canada

[2] Queens Univ, Dept Biochem, Kingston, ON K7L 3N6, Canada

[3] Univ Maine, Dept Biochem Microbiol & Mol Biol, Orono, ME 04469 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999007386

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The absolute requirement of Ca2+ for proteolytic activity is a feature unique to the calpains, a family of heterodimeric cysteine proteases. Conditions are described which give rise to diffraction-quality crystals of m-calpain in two crystal forms, P1 and P2(1). Data have been collected from native crystals of m-calpain in both P1 and P2(1) forms, to 2.6 and 2.15 Angstrom, respectively. Selenomethionine-containing crystals have been grown in both forms, and anomalous data from the P2(1) selenomethionine enzyme provided the location of 17 of the 19 Se atoms in the protein.

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页码：1484 / 1486

页数：3

相关论文

共 16 条

[1] ACTIVE-SITE RESIDUES IN M-CALPAIN - IDENTIFICATION BY SITE-DIRECTED MUTAGENESIS [J].

ARTHUR, JSC ;

GAUTHIER, S ;

ELCE, JS .

FEBS LETTERS, 1995, 368 (03) :397-400

[2] Interaction of aspartic acid-104 and proline-287 with the active site of m-calpain [J].

Arthur, JSC ;

Elce, JS .

BIOCHEMICAL JOURNAL, 1996, 319 :535-541

[3] ALIGNMENT PHYLOGENY OF THE PAPAIN SUPERFAMILY OF CYSTEINE PROTEASES [J].

BERTI, PJ ;

STORER, AC .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 246 (02) :273-283

[4] COMPARISON OF 2 CALCIUM-DEPENDENT PROTEINASES FROM BOVINE HEART [J].

CROALL, DE ;

DEMARTINO, GN .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 788 (03) :348-355

[5] CALCIUM-ACTIVATED NEUTRAL PROTEASE (CALPAIN) SYSTEM - STRUCTURE, FUNCTION, AND REGULATION [J].

CROALL, DE ;

DEMARTINO, GN .

PHYSIOLOGICAL REVIEWS, 1991, 71 (03) :813-847

[6] The effects of truncations of the small subunit on m-calpain activity and heterodimer formation [J].

Elce, JS ;

Davies, PL ;

Hegadorn, C ;

Maurice, DH ;

Arthur, JSC .

BIOCHEMICAL JOURNAL, 1997, 326 :31-38

[7]

Elce JS, 1997, J BIOL CHEM, V272, P11268

[8] RECOMBINANT CALPAIN-II - IMPROVED EXPRESSION SYSTEMS AND PRODUCTION OF A 105A ACTIVE-SITE MUTANT FOR CRYSTALLOGRAPHY [J].

ELCE, JS ;

HEGADORN, C ;

GAUTHIER, S ;

VINCE, JW ;

DAVIES, PL .

PROTEIN ENGINEERING, 1995, 8 (08) :843-848

[9] SELENOMETHIONYL PROTEINS PRODUCED FOR ANALYSIS BY MULTIWAVELENGTH ANOMALOUS DIFFRACTION (MAD) - A VEHICLE FOR DIRECT DETERMINATION OF 3-DIMENSIONAL STRUCTURE [J].

HENDRICKSON, WA ;

HORTON, JR ;

LEMASTER, DM .

EMBO JOURNAL, 1990, 9 (05) :1665-1672

[10] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411