Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection

被引：134

作者：

Kaur, H

Halliwell, B

机构：

[1] Neurodegenerative Disease Research Centre, University of London, King's College, London SW3 6LX, Manresa Road

来源：

BIOCHEMICAL JOURNAL | 1996年 / 318卷

关键词：

D O I：

10.1042/bj3180021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative DNA damage is thought to be an important contributor to cancer development and to be affected by dietary constituents, so its accurate measurement is important. DNA methylation is recognized as an important mechanism affecting gene expression. In the present paper we describe an HPLC-with-electrochemical-detection procedure that allows rapid and sensitive measurement of four oxidized (2,6-diamino-4-hydroxy-5-formamidopyrimidine, 5-hydroxyuracil, 8-hydroxyguanine, 8-hydroxyadenine) and three methylated (7-methylguanine, 1-methylguanine, O-6-methylguanine) bases in acid hydrolysates of DNA. Guanine was also detected, but was clearly separated from the other bases.

引用

页码：21 / 23

页数：3

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