Heterogeneity of metallo-β-lactamases in clinical isolates of Chryseobacterium meningosepticum from Hangzhou, China

被引:19
作者
Chen, GX [1 ]
Zhang, R [1 ]
Zhou, HW [1 ]
机构
[1] Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310009, Peoples R China
关键词
imipenem; resistance; genotypes; phenotypes;
D O I
10.1093/jac/dkl019
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To determine the distribution and heterogeneity of metallo-beta-lactamases (MBLs) responsible for imipenem resistance in Chryseobacterium meningosepticum. Methods: Clinical C. meningosepticum isolates (n = 170) were collected from hospitals in Hangzhou, China. Production of MBLs was investigated by determination of imipenem MICs, and by using both a three-dimensional test and a 2-mercaptopropionic acid inhibitory test. Genes encoding BlaB and GOB MBLs were amplified by PCR, sequenced and compared with genes in GenBank. Results: More than 95% of the 170 isolates showed high (MIC > 16 mg/L) or intermediate resistance to imipenem, but only 94 isolates (55%) were shown phenotypically to produce MBLs (imipenem MIC range, 8-256 mg/L), with MBL genes detected in 93 of these. Among them, 83 isolates had blaB alleles and 65 isolates had bla(GOB) alleles; 38 isolates possessed one MBL gene and 55 isolates contained two genes. The major blaB alleles encoded BlaB-2, -3 and -11, while the major bla(GOB) alleles encoded GOB-2, -4, -8 and -10. MBLs or their genes were not detected in 76 (45%) isolates, including many that were highly resistant to imipenem. Conclusions: High levels and rates of imipenem resistance in C. meningosepticum from Hangzhou often result from the presence of heterogeneous BlaB and/or GOB MBLs, although undefined carbapenem resistance mechanisms also exist. Susceptibility testing and screening for MBLs should be conducted in order to inform effective treatment for C. meningosepticum infections.
引用
收藏
页码:750 / 752
页数:3
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