Production-scale fibronectin nanofibers promote wound closure and tissue repair in a dermal mouse model

被引：79

作者：

Chantre, Christophe O. ^{[1
,2
]}

Campbell, Patrick H. ^{[1
]}

Golecki, Holly M. ^{[1
]}

Buganza, Adrian T. ^{[1
,3
]}

Capulli, Andrew K. ^{[1
]}

Deravi, Leila F. ^{[1
,5
]}

Dauth, Stephanie ^{[1
]}

Sheehy, Sean P. ^{[1
]}

Paten, Jeffrey A. ^{[4
]}

Gledhill, Karl ^{[6
]}

Doucet, Yanne S. ^{[6
]}

Abaci, Hasan E. ^{[6
]}

Ahn, Seungkuk ^{[1
]}

Pope, Benjamin D. ^{[1
,7
]}

Ruberti, Jeffrey W. ^{[4
]}

Hoerstrup, Simon P. ^{[2
]}

Christiano, Angela M. ^{[6
]}

Parker, Kevin Kit ^{[1
]}

机构：

[1] Harvard Univ, John A Paulson Sch Engn & Appl Sci, Wyss Inst Biol Inspired Engn, Dis Biophys Grp, Cambridge, MA 02138 USA

[2] Univ Zurich, Inst Regenerat Med, Zurich, Switzerland

[3] Purdue Univ, Dept Mech Engn, W Lafayette, IN 47907 USA

[4] Northeastern Univ, Dept Bioengn, Boston, MA 02115 USA

[5] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

[6] Columbia Univ, Dept Dermatol, New York, NY 10027 USA

[7] Harvard Med Sch, Beth Israel Deaconess Med Ctr, Boston, MA USA

来源：

BIOMATERIALS | 2018年 / 166卷

基金：

美国国家科学基金会;

关键词：

Fibronectin; Fibrillogenesis; Rotary jet spinning; Nanofiber; Wound healing; Hair follicle; BROWNIAN DYNAMICS SIMULATIONS; SINGLE-POLYMER DYNAMICS; PLASMA FIBRONECTIN; STEM-CELLS; EXTRACELLULAR-MATRIX; SKIN WOUNDS; BIOMATERIALS; REGENERATION; SCAFFOLDS; FETAL;

D O I：

10.1016/j.biomaterials.2018.03.006

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (similar to 1.2 x 10(5) s(-1)) and shear (similar to 3 x 10(5) s(-1)) strain rates in rotary jet spinning (RJS) can drive high throughput Fn fibrillogenesis (similar to 10 mL/min), thus producing nanofiber scaffolds that are used to effectively enhance wound healing. When tested on a full-thickness wound mouse model, Fn nanofiber dressings not only accelerated wound closure, but also significantly improved tissue restoration, recovering dermal and epidermal structures as well as skin appendages and adipose tissue. Together, these results suggest that bioprotein nanofiber fabrication via RJS could set a new paradigm for enhancing wound healing and may thus find use in a variety of regenerative medicine applications. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：96 / 108

页数：13

共 74 条

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