LED Arrays as Cost Effective and Efficient Light Sources for Widefield Microscopy

被引：52

作者：

Albeanu, Dinu F. ^{[1
,2
]}

Soucy, Edward ^{[2
]}

Sato, Tomokazu F. ^{[2
,3
]}

Meister, Markus ^{[1
,2
,3
,4
]}

Murthy, Venkatesh N. ^{[1
,2
,3
,4
]}

机构：

[1] Harvard Univ, Sch Med, Neurosci Program, Boston, MA 02115 USA

[2] Harvard Univ, Ctr Brain Sci, Cambridge, MA USA

[3] Harvard Med Sch, Harvard Biophys Program, Boston, MA USA

[4] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA USA

来源：

PLOS ONE | 2008年 / 3卷 / 05期

关键词：

D O I：

10.1371/journal.pone.0002146

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.

引用

页数：7

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