A method to direct sequence cosmid LAWRIST16 clones

被引：2

作者：

Repetto, M

Ballabio, A

Zollo, M

机构：

[1] TELETHON INST GENET & MED,I-20132 MILAN,ITALY

[2] UNIV SIENA,DEPT BIOL MOL,I-53100 SIENA,ITALY

来源：

DNA SEQUENCE | 1997年 / 7卷 / 3-4期

关键词：

cosmid; method; sequencing;

D O I：

10.3109/10425179709034041

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a new cycle condition method optimized for direct sequencing LAWRIST16 cosmid clones based on dye terminator and dye primer cycle sequencing chemistries. We report a direct comparison of the two most widely used sequencing polymerase enzymes (AmpliTaq(R) FS and Thermosequenase(TM)). The sequencing data obtained is of high quality and we believe this method could be routinely used in large scale sequencing facilities.

引用

页码：229 / &

页数：6

共 12 条

[1] ORDERED SHOTGUN SEQUENCING, A STRATEGY FOR INTEGRATED MAPPING AND SEQUENCING OF YAC CLONES [J].