Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch

被引：56

作者：

Akutsu, Yohei ^{[1
]}

Li, Yu-You ^{[1
,2
]}

Tandukar, Madan ^{[1
]}

Kubota, Kengo ^{[1
]}

Harada, Hideki ^{[1
]}

机构：

[1] Tohoku Univ, Dept Civil & Environm Engn, Sendai, Miyagi 9808579, Japan

[2] Tianjin Inst Urban Construct, Dept Urban & Environm Engn, Tianjin 300384, Peoples R China

来源：

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2008年 / 33卷 / 22期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Seed sludge; Microbial community structure; Stoichiometric equation; Thermophilic hydrogen fermentation; Thermoanaerobacterium;

D O I：

10.1016/j.ijhydene.2008.08.038

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this work, effects of seed sludge on thermophilic hydrogen fermentation were investigated. Five different kinds of sludge were separately inoculated in completely stirred tank reactors (CSTRs), without any pretreatment. The reactors were operated in parallel with starch as substrate under thermophilic condition (55 degrees C) at a hydraulic retention time (HRT) of 24 h for 50 days. Stable hydrogen production was achieved in all reactors. The highest hydrogen yield (2.32 mol H-2/mol glucose) was obtained from the reactor seeded with thermophilically digested activated sludge The stoichiometric equation of hydrogen. fermentation calculated using the data obtained in this study was expressed as follows: starch ([C6H10O5]n) -> 2.32n H-2 + 2.14n CO2 + 0.50n acetate + 0.63n butyrate + 0.11n cell (C5H7NO2). Microbial community structure was analyzed by polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE) and 16S rRNA gene cloning. Close relatives of the Thermoanaerobacterium were found to be the most predominated one in all reactors. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved

引用

页码：6541 / 6548

页数：8

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