Genetic and functional analysis of R5X4 human immunodeficiency virus type 1 envelope glycoproteins derived from two individuals homozygous for the CCR5Δ32 allele

被引：45

作者：

Gray, L

Churchill, MJ

Keane, N

Sterjovski, J

Ellett, AM

Purcell, DFJ

Poumbourios, P

Kol, C

Wang, B

Saksena, NK

Wesselingh, SL

Price, P

French, M

Gabuzda, D

Gorry, PR

机构：

[1] Macfarlane Burnet Inst Med Res & Publ Hlth, Melbourne, Vic 3001, Australia

[2] Univ Melbourne, Dept Microbiol & Immunol, Melbourne, Vic, Australia

[3] Royal Perth Hosp, Dept Clin Immunol & Biochem Genet, Perth, WA, Australia

[4] Univ Western Australia, Sch Surg & Pathol, Perth, WA 6009, Australia

[5] Monash Univ, Dept Med, Melbourne, Vic 3004, Australia

[6] Westmead Millennium Inst, Westmead, NSW, Australia

[7] Dana Farber Canc Inst, Boston, MA 02115 USA

[8] Harvard Univ, Sch Med, Dept Neurol, Boston, MA 02115 USA

来源：

JOURNAL OF VIROLOGY | 2006年 / 80卷 / 07期

关键词：

D O I：

10.1128/JVI.80.7.3684-3691.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5 Delta 32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral blood mononuclear cells (PBMC), but a subset was dually lymphocyte- and macrophage-tropic and used either CCR5 or CXCR4 for entry into PBMC and monocyte-derived macrophages. The persistence of CCR5-using HIV-1 in two CCR5 Delta 32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence.

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页码：3684 / 3691

页数：8

相关论文

共 54 条

[1] A small-molecule, nonpeptide CCR5 antagonist with highly potent and selective anti-HIV-1 activity [J].

Baba, M ;

Nishimura, O ;

Kanzaki, N ;

Okamoto, M ;

Sawada, H ;

Iizawa, Y ;

Shiraishi, M ;

Aramaki, Y ;

Okonogi, K ;

Ogawa, Y ;

Meguro, K ;

Fujino, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (10) :5698-5703

[2] Homozygous Delta 32 deletion of the CCR-5 chemokine receptor gene in an HIV-1-infected patient [J].

Balotta, C ;

Bagnarelli, P ;

Violin, M ;

Ridolfo, AL ;

Zhou, D ;

Berlusconi, A ;

Corvasce, S ;

Corbellino, M ;

Clementi, M ;

Clerici, M ;

Moroni, M ;

Galli, M .

AIDS, 1997, 11 (10) :F67-F71

[3] HIV-1 infection in an individual homozygous for the CCR5 deletion allele [J].

Biti, R ;

French, RF ;

Young, J ;

Bennetts, B ;

Stewart, G ;

Liang, T .

NATURE MEDICINE, 1997, 3 (03) :252-253

[4] The orphan seven-transmembrane receptor Apj supports the entry of primary T-cell-line-tropic and dualtropic human immunodeficiency virus type 1 [J].

Choe, H ;

Farzan, M ;

Konkel, M ;

Martin, K ;

Sun, Y ;

Marcon, L ;

Cayabyab, M ;

Berman, M ;

Dorf, ME ;

Gerard, N ;

Gerard, C ;

Sodroski, J .

JOURNAL OF VIROLOGY, 1998, 72 (07) :6113-6118

[5] The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates [J].

Choe, H ;

Farzan, M ;

Sun, Y ;

Sullivan, N ;

Rollins, B ;

Ponath, PD ;

Wu, LJ ;

Mackay, CR ;

LaRosa, G ;

Newman, W ;

Gerard, N ;

Gerard, C ;

Sodroski, J .

CELL, 1996, 85 (07) :1135-1148

[6] Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CKR5 structural gene [J].

Dean, M ;

Carrington, M ;

Winkler, C ;

Huttley, GA ;

Smith, MW ;

Allikmets, R ;

Goedert, JJ ;

Buchbinder, SP ;

Vittinghoff, E ;

Gomperts, E ;

Donfield, S ;

Vlahov, D ;

Kaslow, R ;

Saah, A ;

Rinaldo, C ;

Detels, R ;

OBrien, SJ .

SCIENCE, 1996, 273 (5283) :1856-1862

[7] Expression cloning of new receptors used by simian and human immunodeficiency viruses [J].

Deng, HK ;

Unutmaz, D ;

KewalRamani, VN ;

Littman, DR .

NATURE, 1997, 388 (6639) :296-300

[8] Identification of a major co-receptor for primary isolates of HIV-1 [J].

Deng, HK ;

Liu, R ;

Ellmeier, W ;

Choe, S ;

Unutmaz, D ;

Burkhart, M ;

DiMarzio, P ;

Marmon, S ;

Sutton, RE ;

Hill, CM ;

Davis, CB ;

Peiper, SC ;

Schall, TJ ;

Littman, DR ;

Landau, NR .

NATURE, 1996, 381 (6584) :661-666

[9] Sensitivity of human immunodeficiency virus type 1 to the fusion inhibitor T-20 is modulated by coreceptor specificity defined by the V3 loop of gp120 [J].

Derdeyn, CA ;

Decker, JM ;

Sfakianos, JN ;

Wu, XY ;

O'Brien, WA ;

Ratner, L ;

Kappes, JC ;

Shaw, GM ;

Hunter, E .

JOURNAL OF VIROLOGY, 2000, 74 (18) :8358-8367

[10] AMD310, a small molecule inhibitor of HIV-1 entry via the CXCR4 co-receptor [J].

Donzella, GA ;

Schols, D ;

Lin, SW ;

Esté, JA ;

Nagashima, KA ;

Maddon, PJ ;

Allaway, GP ;

Sakmar, TP ;

Henson, G ;

De Clercq, E ;

Moore, JP .

NATURE MEDICINE, 1998, 4 (01) :72-77

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