Cigarette smoking induces heat shock protein 70 kDa expression and apoptosis in rat brain: Modulation by bacoside A

被引:68
作者
Anbarasi, K
Kathirvel, G
Vani, G
Jayaraman, G
Devi, CSS
机构
[1] Univ Madras, Dept Biochem, Madras 600025, Tamil Nadu, India
[2] Univ Madras, Dr ALM PG Inst Basic Med Sci, Dept Genet, Madras 600113, Tamil Nadu, India
关键词
Bacopa monniera; cell death; free radicals; oxidative stress; stress proteins;
D O I
10.1016/j.neuroscience.2005.11.029
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Cigarette smoking is associated with the development of several diseases and antioxidants play a major role in the prevention of smoking-related diseases. Apoptosis is suggested as a possible contributing factor in the pathogenesis of smoking-induced toxicity. Therefore the present study was designed to investigate the influence of chronic cigarette smoke exposure on apoptosis and the modulatory effect of bacoside A (triterpenoid saponin isolated from the plant Bacopa monniera) on smoking-induced apoptosis in rat brain. Adult male albino rats of Wistar strain were exposed to cigarette smoke and simultaneously administered with bacoside A (10 mg/kg b.w./day, orally) for a period of 12 weeks. Expression of brain hsp70 was analyzed by Western blotting. Apoptosis was identified by DNA fragmentation, terminal deoxynucleotidyl transferase-mediated deoxy uridine triphosphate nick end labeling (TUNEL) staining and transmission electron microscopy. The results showed that exposure to cigarette smoke induced hsp70 expression and apoptosis as characterized by DNA laddering, increased TUNEL-positive cells and ultrastructural apoptotic features in the brain. Administration of bacoside A prevented expression of hsp70 and neuronal apoptosis during cigarette smoking. We speculate that apoptosis may be responsible for the smoking-induced brain damage and bacoside A can protect the brain from the toxic effects of cigarette smoking. (C) 2005 Published by Elsevier Ltd on behalf of IBRO.
引用
收藏
页码:1127 / 1135
页数:9
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