Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

被引：49

作者：

Lengner, CJ ^{[1
]}

Drissi, H ^{[1
]}

Choi, JY ^{[1
]}

van Wijnen, AJ ^{[1
]}

Stein, JL ^{[1
]}

Stein, GS ^{[1
]}

Lian, JB ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Cell Biol, Worcester, MA 01655 USA

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 114卷 / 1-2期

关键词：

cartilage; sclerotome; somite; development; transcription; AML3; runt homology domain; transgene; beta-galactosidase;

D O I：

10.1016/S0925-4773(02)00050-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 PI promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：167 / 170

页数：4

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