Osteopontin is an autoantigen of the somatostatin cells in human islets: identification by screening random peptide libraries with sera of patients with insulin-dependent diabetes mellitus

被引：22

作者：

Fierabracci, A

Biro, PA

Yiangou, Y

Mennuni, C

Luzzago, A

Ludvigsson, J

Cortese, R

Bottazzo, GF

机构：

[1] Osped Pediat Bambino Gesu, Inst Sci, Direz Sci, I-00165 Rome, Italy

[2] St Bartholomews & Royal London Sch Med & Dent, Dept Immunol, London, England

[3] Ist Ric Biol Mol P Angeletti, Rome, Italy

[4] Linkoping Univ, Fac Hlth Sci, Dept Pediat, Linkoping, Sweden

来源：

VACCINE | 1999年 / 18卷 / 3-4期

关键词：

random peptide libraries (RPLs); insulin-dependent diabetes mellitus (IDDM); autoantigens;

D O I：

10.1016/S0264-410X(99)00204-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Random peptide libraries (RPLs) screening with IDDM sera has identified 5 disease-specific 'mimotopes' displayed on phage (phagotopes). We characterised one phagotope (CH1p), by raising a rabbit antibody against the peptide insert on phage, which was employed in immunohistochemistry, Western blotting and cDNA libraries screening. The CH1p mimotope was detected in somatostatin cells of human islets and experimentally raised anti-osteopontin antibodies or human sera positive for the phagotope, detected a similar subpopulation of islet cells. The screening of cDNA library identified a clone corresponding to human osteopontin. In summary, RPLs proved to be successful in the identification of a novel islet-related autoantigen (osteopentin), whose significance in disease remains to be established. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：342 / 354

页数：13

共 47 条

[1] Toward cell-targeting gene therapy vectors: Selection of cell-binding peptides from random peptide-presenting phage libraries [J].