No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts

被引：8

作者：

Bayer, Monika L. ^{[1
,2
]}

Schjerling, Peter ^{[1
,2
]}

Biskup, Edyta ^{[3
]}

Herchenhan, Andreas ^{[1
,2
]}

Heinemeier, Katja M. ^{[1
,2
]}

Doessing, Simon ^{[1
,2
]}

Krogsgaard, Michael ^{[4
]}

Kjaer, Michael ^{[1
,2
]}

机构：

[1] Univ Copenhagen, Bispebjerg Hosp, Dept Orthoped Surg M, Inst Sports Med, DK-1168 Copenhagen, Denmark

[2] Univ Copenhagen, Fac Hlth Sci, Ctr Healthy Aging, DK-1168 Copenhagen, Denmark

[3] Univ Copenhagen, Bispebjerg Hosp, Dept Dermatol, DK-1168 Copenhagen, Denmark

[4] Univ Copenhagen, Bispebjerg Hosp, Dept Sports Traumatol & Arthroscopy, DK-1168 Copenhagen, Denmark

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2012年 / 133卷 / 05期

基金：

英国医学研究理事会;

关键词：

Extracellular matrix; Tendon; Humoral factors; Collagen; Cell proliferation; GROWTH-FACTOR-BETA; ACTIN STRESS FIBERS; ACHILLES TENDONS; TGF-BETA; FACTOR-I; EXTRACELLULAR-MATRIX; TENOCYTE RESPONSE; BODY-COMPOSITION; SKELETAL-MUSCLE; GENE-EXPRESSION;

D O I：

10.1016/j.mad.2012.02.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-beta 1 (TGF-beta 1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-beta 1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：246 / 254

页数：9

共 81 条

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