Induction of MMP-9 in normal human bronchial epithelial cells by TNF-α via NF-κB-mediated pathway

被引：110

作者：

Hozumi, A ^{[1
]}

Nishimura, Y ^{[1
]}

Nishiuma, T ^{[1
]}

Kotani, Y ^{[1
]}

Yokoyama, M ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Internal Med 1, Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY | 2001年 / 281卷 / 06期

关键词：

matrix metalloproteinase-2; matrix metalloproteinase-9; tumor necrosis factor-alpha; nuclear factor-kappa B; proinflammatory cytokine; airway inflammation;

D O I：

10.1152/ajplung.2001.281.6.L1444

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

In this study, we determined whether the proinflammatory cytokines tumor necrosis factor (TNF)-alpha and interleukin-1 beta contribute to the regulation of matrix metalloproteinase (MMP)-9 in human bronchial epithelial cells and whether the induction of MMP-9 is regulated by the transcription factor nuclear factor (NF)-kappaB. We demonstrated that TNF-alpha induced MMP-9 at both the protein and mRNA levels in human bronchial epithelial cells and that interleukin-1 beta did not. In contrast, induction of the tissue inhibitor of metalloproteinase-1 by TNF-alpha was less than that of interleukin-1b. Increased expression of MMP-9 and NF-kappaB activation induced by TNF-alpha were inhibited by pyrrolidine dithiocarbamate and N-acetyl-L-cysteine but were not inhibited by curcumin. These results suggest that TNF-alpha induces the expression of MMP-9 in human bronchial epithelial cells and that this induction is mediated via the NF-kappaB-mediated pathway.

引用

页码：L1444 / L1452

页数：9

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