Photoprotective effect of Undaria crenata against ultraviolet B-induced damage to keratinocytes

被引：15

作者：

Hyun, Yu Jae ^{[1
]}

Piao, Mei Jing ^{[1
]}

Ko, Mi Hee ^{[2
]}

Lee, Nam Ho ^{[3
]}

Kang, Hee Kyoung ^{[1
]}

Yoo, Eun Sook ^{[1
]}

Koh, Young Sang ^{[1
]}

Hyun, Jin Won ^{[1
]}

机构：

[1] Jeju Natl Univ, Sch Med, Cheju 690756, South Korea

[2] Jeju Technopk, Jeju Biodivers Res Inst, Cheju 699943, South Korea

[3] Jeju Natl Univ, Dept Chem, Coll Nat Sci, Cheju 690756, South Korea

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2013年 / 116卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Human keratinocytes; Oxidative stress; Reactive oxygen species; Ultraviolet B; Undaria crenata; UVB-INDUCED APOPTOSIS; ANTITUMOR-ACTIVITY; ENDOTHELIAL-CELLS; HAIRLESS MICE; SKIN-CANCER; DNA-DAMAGE; SKH-1; MICE; PINNATIFIDA; BROWN; CARCINOGENESIS;

D O I：

10.1016/j.jbiosc.2013.02.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Chronic exposure of the skin to ultraviolet B (UVB) radiation induces oxidative stress, which plays a crucial role in the induction of skin cancer. The brown alga Undaria crenata is a potential source of antioxidant and anti-apoptotic compounds due to its capacity to produce protective compounds against environmental factors, including UV radiation. The aim of this study was to investigate the photoprotective properties of an U. crenata ethanol extract (UCE) against UVB-induced cell damage in human HaCaT keratinocytes. UCE exhibited absorbing effect of UVB (280-320 nm) and scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species induced by hydrogen peroxide and UVB rays. Furthermore, electron spin resonance spectrometry revealed the significant scavenging effect of UCE against superoxide anion and hydroxyl radical. UCE reduced UVB-induced apoptosis, as shown by a decrease in apoptotic bodies and nuclear and DNA fragmentation, resulting in the recovery of cell viability. UCE also decreased the degree of UVB-induced oxidative stress to lipids, proteins, and DNA as shown by a decrease in 8-isoprostane level, protein carbonylation and DNA tails. These results suggest that UCE protects human keratinocytes against UVB-induced oxidative stress. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：256 / 264

页数：9

共 44 条

[1]

Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways [J].