Localized XId3 mRNA activation in Xenopus embryos by cytoplasmic polyadenylation

被引：9

作者：

Afouda, AB

Reynaud-Deonauth, S

Mohun, T

Spohr, G ^{[1
]}

机构：

[1] Univ Geneva, Dept Cell Biol, CH-1211 Geneva 4, Switzerland

[2] Natl Inst Med Res, Lab Dev Biochem, London NW7 1AA, England

来源：

MECHANISMS OF DEVELOPMENT | 1999年 / 88卷 / 01期

关键词：

Xenopus development; localization; inhibition; cytoplasmic polyadenylation; palindrome CPE; oocyte maturation; GVBD; post-transcriptional regulation; Id2; sequence; Id3; maternal mRNA; galactosidase; in situ; epithelial sensorial layer; animal cap; gasrtrula; embryo; HLH bHLH transcription factor; 3 ' UTR translation; control prepattern axis;

D O I：

10.1016/S0925-4773(99)00166-5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Xenopus development, during meiosis and cleavage, the extent of polyadenylation plays a central role in regulating the expression of transcripts and this is mediated by cis regulatory cytoplasmic polyadenylation elements (CPE) in the 3'-UTRs. We have identified a palindromic CPE in the mRNA of Xenopus Id3 which is conserved in the Id genes from other vertebrates. It promotes cytoplasmic polyadenylation and is negatively regulated by sequences further upstream in the 3'-UTR. This palindromic CPE promotes polyadenylation in both the epithelial and sensorial layers of the dorsal ectoderm in early embryos, but association with the upstream negative element blocks this effect in the epithelial layer. The asymmetric polyadenylation may be important for establishing a prepattern of transcriptional regulators. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：15 / 31

页数：17

共 42 条

[1] A MATERNAL TAIL OF POLY(A) - THE LONG AND THE SHORT OF IT [J].