Development of an optimal bacterial medium based on the growth inhibition assay with Vibrio fischeri

被引：22

作者：

Gellert, G ^{[1
]}

Stommel, A ^{[1
]}

Trujillano, AB ^{[1
]}

机构：

[1] Staatliches Umweltamt Koln Aussenstelle Bonn, D-53113 Bonn, Germany

来源：

CHEMOSPHERE | 1999年 / 39卷 / 03期

关键词：

Vibrio fischeri; bacterial toxicity; growth inhibition; medium composition; yeast extract;

D O I：

10.1016/S0045-6535(99)00002-8

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Chronic toxicity level of chemicals to bacteria can depend on composition and concentration of medium ingredients. This was demonstrated by means of a growth rate inhibition test with the marine bacterium V. fischeri. In a minimal medium (following the validity criterion of achieving a least cell multiplication rate) containing only yeast extract as organic nutrient component, V. fischeri was to Cu2+ 11,9 times, to Hg2+ 3 times and to Zn2+ 2,8 times more sensitive than in the complete defined medium. Obviously yeast extract did not interfere greatly in the complexation mechanisms. The detection limits of the toxicity of Cd2+, 3,5-dichorophenol and nitrobenzene were not influenced by the substrate. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：467 / 476

页数：10

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