Mucin-positive epithelial mesotheliomas: A histochemical, immunohistochemical, and ultrastructural comparison with mucin-producing pulmonary adenocarcinomas

被引:57
作者
Hammar, SP
Bockus, DE
Remington, FL
Rohrbach, KA
机构
[1] Diagnostic Specialties Laboratory, Bremerton, WA
[2] Diagnostic Specialties Laboratory, Bremerton, WA 98310
关键词
epithelial mesothelioma; histochemistry; hyaluronic acid proteoglycan crystals; immunohistochemistry electron microscopy; mucin; pulmonary adenocarcinoma;
D O I
10.3109/01913129609016331
中图分类号
TH742 [显微镜];
学科分类号
摘要
Pathologists routinely use histochemistry, immunohistochemistry, and electron microscopy to differentiate epithelial mesotheliomas from pulmonary adenocarcinomas. Epithelial mesotheliomas are usually mucicarmine-, PAS-diastase, and carcinoembryonic antigen-negative, whereas about 60-75% of pulmonary adenocarcinomas are mucicarmine- and PAS-diastase-positive, and about 90% express polyclonal carcinoembryonic antigen. During a pathologic evaluation of pleural neoplasms between 1975 and 1990, 10 epithelial mesotheliomas were identified that were mucicarmine- and in some instances PAS-diastase-positive (diagnosis of mesothelioma confirmed by ultrastructural examination), with four mesotheliomas focally expressing carcinoembryonic antigen. The mucicarmine, PAS-diastase, and carcinoembryonic antigen staining were usually eradicated or reduced in intensity by pretreatment of the tissue sections with hyaluronidase, suggesting that hyaluronic acid was responsible for the positive mucin reactions. In three cases the epithelial mesotheliomas showed focal regions of mucicarmine, PAS-d-, and Alcian blue-hyaluronidase-resistant staining. In contrast, 10 mucicarmine-, PAS-diastase-, Alcian blue-, and carcinoembryonic antigen-positive pulmonary adenocarcinomas were not affected by hyaluronidase pretreatment of the tissue. Besides the usual ultrastructural features of well- to moderately well-differentiated epithelial mesotheliomas, the mucin-positive epithelial mesotheliomas often showed medium-electron-dense secretory material covering the microvilli, aggregates of medium electron-dense material in association with the microvilli, producing an ultrastructural morphology that has been observed only in epithelial mesotheliomas.
引用
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页码:293 / 325
页数:33
相关论文
共 43 条
  • [1] ALBERNET R, 1979, REV FR MAL RESPIR, V7, P243
  • [2] [Anonymous], MSA B
  • [3] BENJAMIN CJ, 1982, AM J MED TECHNOL, V48, P905
  • [4] MULTIPLE-MARKER IMMUNOHISTOCHEMICAL PHENOTYPES DISTINGUISHING MALIGNANT PLEURAL MESOTHELIOMA FROM PULMONARY ADENOCARCINOMA
    BROWN, RW
    CLARK, GM
    TANDON, AK
    ALLRED, DC
    [J]. HUMAN PATHOLOGY, 1993, 24 (04) : 347 - 354
  • [5] DESMOSOMES OF EPITHELIAL MALIGNANT MESOTHELIOMA
    BURNS, TR
    JOHNSON, EH
    CARTWRIGHT, J
    GREENBERG, SD
    [J]. ULTRASTRUCTURAL PATHOLOGY, 1988, 12 (04) : 385 - 388
  • [6] Caterson B, 1987, BIOL PROTEOGLYCANS, P1
  • [7] COLEMAN M, 1989, PATHOL ANNU, V24, P303
  • [8] HISTOCHEMICAL METHOD OF DIFFERENTIATING LOWER GASTROINTESTINAL-TRACT MUCIN FROM OTHER MUCINS IN PRIMARY OR METASTATIC TUMORS
    CULLING, CFA
    REID, PE
    BURTON, JD
    DUNN, WL
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 1975, 28 (08) : 656 - 658
  • [9] PLEURAL MESOTHELIOMA OF EPITHELIAL TYPE AND PULMONARY ADENOCARCINOMA - AN ULTRASTRUCTURAL AND CYTOCHEMICAL COMPARISON
    DEWAR, A
    VALENTE, M
    RING, NP
    CORRIN, B
    [J]. JOURNAL OF PATHOLOGY, 1987, 152 (04) : 309 - 316
  • [10] ERNST CS, 1980, LAB INVEST, V42, P113