Cytochrome P450 isozymes involved in the metabolism of phenol, a benzene metabolite

被引：24

作者：

Powley, MW ^{[1
]}

Carlson, GP ^{[1
]}

机构：

[1] Purdue Univ, Sch Hlth Sci, W Lafayette, IN 47907 USA

来源：

TOXICOLOGY LETTERS | 2001年 / 125卷 / 1-3期

关键词：

phenol; benzene; liver; lung; CYP2E1; CYP2F2;

D O I：

10.1016/S0378-4274(01)00441-6

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Benzene is an occupational and environmental toxicant. The major health concern for humans is acute myelogenous leukemia. To exert its toxic effects, benzene must be metabolized by cytochrome P450 to phenol and subsequently to catechol and hydroquinone. Previous research has implicated CYP2E1 in the metabolism of phenol. In this study the cytochrome P450 isozymes involved in the metabolism of phenol were examined in hepatic and pulmonary microsomes utilizing chemical inhibitors of CYP2E1, CYP2B, and CYP2F2 and using CYP2E1 knockout mice. CYP2E1 was found to be responsible for only similar to 50% of 20 muM phenol metabolism in the liver. This suggests another isozyme(s) is involved in hepatic phenol metabolism. In pulmonary microsomes both CYP2E1 and CYP2F2 were significantly involved. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：117 / 123

页数：7

共 27 条

[1] Effects of inhibitors of CYP1A and CYP2B on styrene metabolism in mouse liver and lung microsomes [J].