Value of enterobacterial repetitive intergenic consensus PCR for study of Pasteurella multocida strains isolated from mouths of dogs

被引:17
作者
Loubinoux, J
Lozniewski, A
Lion, C
Garin, D
Weber, M
Le Faou, AE
机构
[1] CHU Nancy Brabois, Virol Lab, F-54511 Vandoeuvre Nancy, France
[2] CHU Nancy, Bacteriol Lab, F-54035 Nancy, France
[3] HLA Legouest, Serv Biol Med, F-57000 Metz, France
关键词
D O I
10.1128/JCM.37.8.2488-2492.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armee de Terre, Suippes, France) were studied by use of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and restriction fragment length polymorphism (RFLP) techniques. Both techniques showed genomic heterogeneity of the strains studied. However, RFLP was more discriminatory than ERIC-PCR for differentiating P. multocida strains. All but three pairs of strains were discriminated by RFLP, suggesting a limited circulation of strains between these dogs living in proximity. Although ERIC-PCR is easier and faster to perform, it cannot be recommended for epidemiological studies of P, multocida strains.
引用
收藏
页码:2488 / 2492
页数:5
相关论文
共 31 条
[1]  
CARTER GR, 1955, AM J VET RES, V16, P481
[2]   Validation of random amplified polymorphic DNA assays by ribotyping as tools for epidemiological surveys of Pasteurella from animals [J].
ChaslusDancla, E ;
LesageDescauses, MC ;
LeroySetrin, S ;
Martel, JL ;
Coudert, P ;
Lafont, JP .
VETERINARY MICROBIOLOGY, 1996, 52 (1-2) :91-102
[3]   EPIDEMIOLOGIC TYPING OF STENOTROPHOMONAS (XANTHOMONAS) MALTOPHILIA BY PCR [J].
CHATELUT, M ;
DOURNES, JL ;
CHABANON, G ;
MARTY, N .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (04) :912-914
[4]   MEASURES OF THE AMOUNT OF ECOLOGIC ASSOCIATION BETWEEN SPECIES [J].
DICE, LR .
ECOLOGY, 1945, 26 (03) :297-302
[5]  
DONNIO PY, 1994, MANUAL CLIN BACTERIO, P1371
[6]   PCR-MEDIATED DNA TYPING OF CAMPYLOBACTER-JEJUNI ISOLATED FROM PATIENTS WITH RECURRENT INFECTIONS [J].
ENDTZ, HP ;
GIESENDORF, BAJ ;
VANBELKUM, A ;
LAUWERS, SJM ;
JANSEN, WH ;
QUINT, WGV .
RESEARCH IN MICROBIOLOGY, 1993, 144 (09) :703-708
[7]   MOLECULAR EPIDEMIOLOGY OF INFECTIONS DUE TO ENTEROBACTER-AEROGENES - IDENTIFICATION OF HOSPITAL OUTBREAK-ASSOCIATED STRAINS BY MOLECULAR TECHNIQUES [J].
GEORGHIOU, PR ;
HAMILL, RJ ;
WRIGHT, CE ;
VERSALOVIC, J ;
KOEUTH, T ;
WATSON, DA ;
LUPSKI, JR .
CLINICAL INFECTIOUS DISEASES, 1995, 20 (01) :84-94
[8]   BITE WOUNDS AND INFECTION [J].
GOLDSTEIN, EJC .
CLINICAL INFECTIOUS DISEASES, 1992, 14 (03) :633-638
[9]   FOWL CHOLERA - GEL-DIFFUSION PRECIPITIN TEST FOR SEROTYPING PASTEURELLA-MULTOCIDA FROM AVIAN SPECIES [J].
HEDDLESTON, KL ;
REBERS, PA ;
GALLAGHER, JE .
AVIAN DISEASES, 1972, 16 (04) :925-+
[10]   RESISTANCE TO ANTIMICROBIAL AGENTS AND PREVALENCE OF R-PLASMIDS IN PASTEURELLA-MULTOCIDA FROM TURKEYS [J].
HIRSH, DC ;
HANSEN, LM ;
DORFMAN, LC ;
SNIPES, KP ;
CARPENTER, TE ;
HIRD, DW ;
MCCAPES, RH .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (05) :670-673