Assembly of sugars on polystyrene plates: a new facile microarray fabrication technique

被引：17

作者：

Fazio, F

Bryan, MC

Lee, HK

Chang, A

Wong, CH

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

TETRAHEDRON LETTERS | 2004年 / 45卷 / 12期

关键词：

microarray; oligosaccharides; detection by ESI-MS;

D O I：

10.1016/j.tetlet.2004.01.159

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The work presented herein is a new noncovalent glycoarray assembly method for microplates created by simply mixing together an isocyanate-containing C-14-hydrocarbon and an amine-containing carbohydrate. 2-Aminoethyl-beta-D-galactopyranoside (1) was utilized in model studies and product formation was detected by both ESI-MS and lectin binding. The method has been further extended to array complex carbohydrates. (C) 2004 Elsevier Ltd. All rights reserved.

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收藏

页码：2689 / 2692

页数：4

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