BRAF and PIK3CA genes are somatically mutated in hepatocellular carcinoma among patients from South Italy

被引：71

作者：

Colombino, M. ^{[2
]}

Sperlongano, P. ^{[3
]}

Izzo, F. ^{[4
]}

Tatangelo, F. ^{[4
]}

Botti, G. ^{[4
]}

Lombardi, A. ^{[1
]}

Accardo, M. ^{[5
]}

Tarantino, L. ^{[6
]}

Sordelli, I. ^{[3
]}

Agresti, M. ^{[3
]}

Abbruzzese, A. ^{[1
]}

Caraglia, M. ^{[1
]}

Palmieri, G. ^{[2
]}

机构：

[1] Univ Naples 2, Dept Biochem & Biophys, I-80138 Naples, Italy

[2] Natl Res Council CNR, Inst Biomol Chem, Sassari, Italy

[3] Univ Naples 2, Dept Surg, I-80138 Naples, Italy

[4] Natl Canc Inst Fdn G Pascale, Naples, Italy

[5] Univ Naples 2, Dept Publ & Prevent Med, I-80138 Naples, Italy

[6] S Giovanni di Dio Hosp, Dept Surg, Naples, Italy

来源：

CELL DEATH & DISEASE | 2012年 / 3卷

关键词：

hepatocellular carcinoma; cancer genes; mutation analysis; patients' molecular classification; HUMAN CANCER; MUTATIONS; MELANOMA; SORAFENIB; PATHWAY;

D O I：

10.1038/cddis.2011.136

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Poor data have been previously reported about the mutation rates in K-RAS, BRAF, and PIK3CA genes among patients with hepatocellular carcinoma (HCC). Here we further elucidated the role of these genes in pathogenesis of primary hepatic malignancies. Archival tumour tissue from 65 HCC patients originating from South Italy were screened for mutations in these candidate genes by direct sequencing. Overall, oncogenic mutations were detected in 15 (23%) patients for BRAF gene, 18 (28%) for PIK3CA gene, and 1 (2%) for K-RAS gene. Using statistical analysis, BRAF mutations were significantly correlated with the presence of either multiple HCC nodules (P = 0.021) or higher proliferation rates (P = 0.034). Although further extensive screenings are awaited in HCC patients among different populations, our findings clearly indicated that mutational activation of both BRAF and PIK3CA genes does contribute to hepatocellular tumorigenesis at somatic level in Southern Italian population. Cell Death and Disease (2012) 3, e259; doi:10.1038/cddis.2011.136; published online 19 January 2012

引用

页码：e259 / e259

页数：4

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