Caveolin-1 up-regulates ST6Gal-I to promote the adhesive capability of mouse hepatocarcinoma cells to fibronectin via FAK-mediated adhesion signaling

被引：33

作者：

Yu, Shengjin ^{[1
,2
]}

Zhang, Lijun ^{[1
]}

Li, Nanyang ^{[1
]}

Fan, Jianhui ^{[1
]}

Liu, Linhua ^{[1
]}

Zhang, Jianing ^{[1
]}

Wang, Shujing ^{[1
]}

机构：

[1] Dalian Med Univ, Dept Biochem, Inst Glycobiol, Dalian 116044, Peoples R China

[2] Eastern Liaoning Univ, Coll Med, Dept Basic Med Sci, Dandong 118000, Liaoning Provin, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 427卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Caveolin-1; ST6Gal-I; Glycosylation; Adhesion; Hepatocarcinoma; EXPRESSION; SIALYLTRANSFERASE; GLYCOSYLATION; CARCINOMA; MIGRATION; CANCER; LOCALIZATION; METASTASIS; MECHANISMS; INVASION;

D O I：

10.1016/j.bbrc.2012.09.086

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Caveolin-1 is a major structural protein of caveolae and plays important functions in tumorigenesis and development. Hca-F and Hepa1-6 are mouse hepatocarcinoma cell lines with high and low malignant potential, respectively. Our previous studies revealed that caveolin-1 promoted cell invasion by up-regulating the glycosylation of matrix metalloproteinase inducer CD147 of Hepa1-6 and Hca-F cells. However, the roles of caveolin-1 in cell-ECM adhesion and the mechanisms involved remain unknown. This study showed that caveolin-1 overexpression in Hepa1-6 cells up-regulated sialyltransferase ST6Gal-I expression and activated FAK-mediated adhesion signaling, and down-regulation of ST6Gal-I attenuated caveolin-1-induced increase in the adhesive ability of Hepa1-6 cells to fibronectin. Conversely, caveolin-1 knockdown in Hca-F cells inhibited ST6Gal-I expression and FAK signaling-mediated cell adhesion to fibronectin. Re-expression of wild-type caveolin-1 or ST6Gal-I rescued the decreased ST6Gal-I expression and adhesion of Hca-F cells caused by caveolin-1 silencing. Further studies indicated that caveolin-1 might regulate ST6Gal-I expression through caveolin-1 scaffolding domain. Taken together, these results demonstrate for the first time that caveolin-1 can up-regulate ST6Gal-I expression and further contribute to promoting mouse hepatocarcinoma cell adhesion to fibronectin by activating FAK-mediated adhesion signaling. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：506 / 512

页数：7

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