Totally in vitro protein selection using mRNA-protein fusions and ribosome display

被引：90

作者：

Roberts, RW ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1999年 / 3卷 / 03期

关键词：

D O I：

10.1016/S1367-5931(99)80042-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both chemists and biochemists have great interest in creating peptides and proteins with desired structure, recognition and catalytic properties. Recently, two techniques have been developed that afford functional selection of proteins entirely in vitro: mRNA-protein fusions, and ribosome display. Improvements in both methods have allowed peptide and protein libraries of unprecedented size (10(11)-10(13) different members) to be generated and screened for function.

引用

页码：268 / 273

页数：6

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