Adenovirus small e1a alters global patterns of histone modification

被引：168

作者：

Horwitz, Gregory A. ^{[1
]}

Zhang, Kangling ^{[2
]}

McBrian, Matthew A. ^{[3
]}

Grunstein, Michael ^{[3
]}

Kurdistani, Siavash K. ^{[3
]}

Berk, Arnold J. ^{[1
,4
]}

机构：

[1] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

[2] Loma Linda Univ, Mass Spectrometry Facil, Dept Biochem, Sch Med, Loma Linda, CA 92350 USA

[3] Univ Calif Los Angeles, Dept Biol Chem, David Geffen Sch Med, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

来源：

SCIENCE | 2008年 / 321卷 / 5892期

关键词：

D O I：

10.1126/science.1155544

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adenovirus small early region 1a ( e1a) protein drives cells into S phase by binding RB family proteins and the closely related histone acetyl transferases p300 and CBP. The interaction with RB proteins displaces them from DNA- bound E2F transcription factors, reversing their repression of cell cycle genes. However, it has been unclear how the e1a interaction with p300 and CBP promotes passage through the cell cycle. We show that this interaction causes a threefold reduction in total cellular histone H3 lysine 18 acetylation ( H3K18ac). CBP and p300 are required for acetylation at this site because their knockdown causes specific hypoacetylation at H3K18. SV40 T antigen also induces H3K18 hypoacetylation. Because global hypoacetylation at this site is observed in prostate carcinomas with poor prognosis, this suggests that processes resulting in global H3K18 hypoacetylation may be linked to oncogenic transformation.

引用

页码：1084 / 1085

页数：2

共 17 条

[1] SV40 large T antigen targets multiple cellular pathways to elicit cellular transformation [J].

Ahuja, D ;

Sáenz-Robles, MT ;

Pipas, JM .

ONCOGENE, 2005, 24 (52) :7729-7745

[2] Recent lessons in gene expression, cell cycle control, and cell biology from adenovirus [J].

Berk, AJ .

ONCOGENE, 2005, 24 (52) :7673-7685

[3] E2F-associated chromatin modifiers and cell cycle control [J].

Blais, Alexandre ;

Dynlacht, Brian D. .

CURRENT OPINION IN CELL BIOLOGY, 2007, 19 (06) :658-662

[4] CtBP, an unconventional transcriptional corepressor in development and oncogenesis [J].

Chinnadurai, G .

MOLECULAR CELL, 2002, 9 (02) :213-224

[5] Epigenetic reprogramming by adenovirus e1a [J].

Ferrari, Roberto ;

Pellegrini, Matteo ;

Horwitz, Gregory A. ;

Xie, Wei ;

Berk, Arnold J. ;

Kurdistani, Siavash K. .

SCIENCE, 2008, 321 (5892) :1086-1088

[6] The p400 complex is an essential E1A transformation target [J].

Fuchs, M ;

Gerber, J ;

Drapkin, R ;

Sif, S ;

Ikura, T ;

Ogryzko, V ;

Lane, WS ;

Nakatani, Y ;

Livingston, DM .

CELL, 2001, 106 (03) :297-307

[7] ADENOVIRUS TYPE-5 EARLY GENE-FUNCTION REGULATES EXPRESSION OF OTHER EARLY VIRAL GENES [J].

JONES, N ;

SHENK, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (08) :3665-3669

[8] Chromatin modifications and their function [J].

Kouzarides, Tony .

CELL, 2007, 128 (04) :693-705

[9] Structure of the retinoblastoma protein bound to adenovirus E1A reveals the molecular basis for viral oncoprotein inactivation of a tumor suppressor [J].

Liu, Xin ;

Marmorstein, Ronen .

GENES & DEVELOPMENT, 2007, 21 (21) :2711-2716

[10] COMPLETE TRANSFORMATION BY ADENOVIRUS-2 REQUIRES BOTH E1A-PROTEINS [J].

MONTELL, C ;

COURTOIS, G ;

ENG, C ;

BERK, A .

CELL, 1984, 36 (04) :951-961

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