Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation

被引：110

作者：

Kinto, N

Iwamoto, M

EnomotoIwamoto, M

Noji, S

Ohuchi, H

Yoshioka, H

Kataoka, H

Wada, Y

Yuhao, G

Takahashi, HE

Yoshiki, S

Yamaguchi, A

机构：

[1] SHOWA UNIV,SCH DENT,DEPT ORAL PATHOL,SHINAGAWA KU,TOKYO 142,JAPAN

[2] SHOWA UNIV,SCH DENT,DEPT ORTHODONT,SHINAGAWA KU,TOKYO 142,JAPAN

[3] OSAKA UNIV,FAC DENT,DEPT ANAT & CELL BIOL,SUITA,OSAKA 565,JAPAN

[4] OSAKA UNIV,FAC DENT,DEPT BIOCHEM,SUITA,OSAKA 565,JAPAN

[5] UNIV TOKUSHIMA,FAC ENGN,DEPT BIOL SCI & TECHNOL,TOKUSHIMA 770,JAPAN

[6] NIIGATA UNIV,SCH MED,DEPT ORTHOPED SURG,NIIGATA 951,JAPAN

来源：

FEBS LETTERS | 1997年 / 404卷 / 2-3期

关键词：

Sonic hedgehog; osteoblast differentiation; bone formation;

D O I：

10.1016/S0014-5793(97)00014-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation. (C) 1997 Federation of European Biochemical Societies.

引用

页码：319 / 323

页数：5

共 25 条

[1] Human osteogenic protein-1 induces chondroblastic, osteoblastic, and/or adipocytic differentiation of clonal murine target cells [J].