Circulating extracellular vesicles in the aging process: impact of aerobic exercise

被引：83

作者：

Bertoldi, Karine ^{[1
]}

Cechinel, Laura Reck ^{[1
]}

Schallenberger, Bruna ^{[2
]}

Corssac, Giana Blume ^{[1
,4
]}

Davies, Samuel ^{[3
]}

Kuelkamp Guerreiro, Irene Clemes ^{[3
]}

Bello-Klein, Adriane ^{[1
,4
]}

Araujo, Alex Sander R. ^{[1
,4
]}

Siqueira, Ionara Rodrigues ^{[1
,2
,5
]}

机构：

[1] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Biol Fisiol, Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Farmacol, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencias Farmaceut, Fac Farm, Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Lab Fisiol Cardiovasc & Especies Reat Oxigenio, Dept Fisiol, Inst Ciencias Basicas Saude, Porto Alegre, RS, Brazil

[5] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Lab Neuropsicofarmacol, Dept Farmacol, Rua Sarmento Leite 500,Sala 313, BR-90050170 Porto Alegre, RS, Brazil

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2018年 / 440卷 / 1-2期

关键词：

Circulating extracellular vesicles; Exosomes; Aging; Reactive species content; Rats; Treadmill exercise; PLATELET-DERIVED EXOSOMES; APP/PS1 TRANSGENIC MICE; AGE-RELATED-CHANGES; OXIDATIVE STRESS; ALZHEIMERS-DISEASE; RAT HIPPOCAMPUS; ACETYLCHOLINESTERASE ACTIVITY; ISCHEMIA-REPERFUSION; PHYSICAL-ACTIVITY; OXIDASE ACTIVITY;

D O I：

10.1007/s11010-017-3160-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Our aim was to investigate transitory and delayed exercise effects on serum extracellular vesicles (EVs) in aging process. Male Wistar rats of 3-, 21-, and 26-month old were allocated into exercised and sedentary groups. The exercise protocol consisted in a daily moderate treadmill exercise (20 min daily during 2 weeks). Trunk blood was collected 1 and 18 h after the last exercise session, and circulating EVs were obtained. CD63 levels and acetylcholinesterase (AChE) activity were used as markers of exosome, a subtype of EVs. In addition, the quantification of amyloid-beta (A beta) levels and the oxidative status parameters, specifically reactive species content, superoxide dismutase (SOD) activity, and SOD1 content were evaluated. Aged rats showed reduced CD63 levels and increased AChE activity in circulating exosomes compared to young ones. Moreover, higher reactive species levels were found in circulating EVs of aged rats. Delayed exercise effects were observed on peripheral EVs, since CD63, reactive species content, and AChE activity were altered 18 h after the last exercise session. Our results suggest that the healthy aging process can modify circulating EVs profile, and exercise-induced beneficial effects may be related to its modulation on EVs.

引用

页码：115 / 125

页数：11

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