Independent dynamic regulation of histone phosphorylation and acetylation during immediate-early gene induction

被引：165

作者：

Thomson, S ^{[1
]}

Clayton, AL ^{[1
]}

Mahadevan, LC ^{[1
]}

机构：

[1] Univ Oxford, Dept Biochem, Nucl Signalling Lab, Oxford OX1 3QU, England

来源：

MOLECULAR CELL | 2001年 / 8卷 / 06期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S1097-2765(01)00404-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Induction of c-fos and c-jun is associated with phosphoacetylation of histone H3 and acetylation of histone H4. Upon induction, a large population of nucleosomes becomes highly acetylated on histones H3 and H4, whereas a much smaller population of comparable nucleosomes at similar positions along the gene becomes phosphoacetylated. Inhibiting histone H3 phosphorylation with kinase inhibitors does not measurably alter the enhanced acetylation of these nucleosomes. Finally, whereas H3 phosphorylation is a MAP kinase-mediated inducible event, we found acetylation to be continuously turning over by the targeted action of HATs and HDACs in the absence of any stimulation or gene transcription. These studies suggest that phosphorylation and acetylation are independently and dynamically regulated at these genes and reveal the complexity of multiple histone modifications at immediate-early gene chromatin.

引用

页码：1231 / 1241

页数：11

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