A probing feeding strategy for Escherichia coli cultures

被引：33

作者：

Åkesson, M

Hagander, P

Axelsson, JP

机构：

[1] Lund Inst Technol, Dept Automat Control, SE-22100 Lund, Sweden

[2] Pharmacia & Upjohn Inc, Proc R&D, SE-11287 Stockholm, Sweden

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 08期

关键词：

acetate; dissolved oxygen; Escherichia coli; feedback control; glucose feeding;

D O I：

10.1023/A:1008906517033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.

引用

页码：523 / 528

页数：6

共 9 条

[1]

AKESSON M, 1999, IN PRESS BIOTECHNOL

[2] ARE GROWTH-RATES OF ESCHERICHIA-COLI IN BATCH CULTURES LIMITED BY RESPIRATION [J].