Purification, structure, lipid lowering and liver protecting effects of polysaccharide from Lachnum YM281

被引：117

作者：

Qiu, Tao ^{[1
]}

Ma, Xiaojing ^{[1
]}

Ye, Ming ^{[1
]}

Yuan, Ruyue ^{[1
]}

Wu, Yanna ^{[1
]}

机构：

[1] Hefei Univ Technol, Coll Biotechnol & Food Engn, Microbial Resources & Applicat Lab, Hefei 230009, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 98卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Lachnum YM281; Extracellular polysaccharide; Structure; Lipid lowering; Liver protecting; WATER-SOLUBLE POLYSACCHARIDE; FRUITING BODIES; HYPOGLYCEMIC ACTIVITY; PLEUROTUS-OSTREATUS; GANODERMA-LUCIDUM; MOLECULAR-WEIGHT; RATS; CONFORMATION; FIBER;

D O I：

10.1016/j.carbpol.2013.07.014

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The extracellular polysaccharide (LEP) produced by Lachnum YM281 was obtained from the fermentation broth, and LEP-lb with molecular weight of 4.02 x 10(4) Da was separated and sequentially purified through DEAE-cellulose 52 column chromatography and Sepharose CL-6B column chromatography. GC-MS, IR and NMR (H-1, C-13) spectroscopy analysis indicated that the repeat unit of LEP-1b was: beta-D-Glcp 1 down arrow 3 beta-D-Glcp 1 down arrow 6 -> 3)-beta-D-Glcp-(1 -> 3)-beta-D-Glcp-(1 -> 3)-beta-D-Glcp-(1 -> 3)-beta-D-G;cp-(1 -> 3)-beta-D-Glcp-(1 -> The effects of LEP-lb on the serum lipids, liver lipids levels and aminotransferase activities of model mice with hyperlipidemic fatty live were studied, and the results showed that LEP-1b had strong lipid lowering and liver protecting effects on mice with hyperlipidemic fatty live. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：922 / 930

页数：9

共 32 条

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Structural features of immunologically active polysaccharides from Ganoderma lucidum [J].