hTERT gene dosage correlates with telomerase activity in human lung cancer cell lines

被引：45

作者：

Saretzki, G

Petersen, S

Petersen, I

Kölble, K

von Zglinicki, T

机构：

[1] Newcastle Univ, Newcastle Gen Hosp, Wolfson Res Ctr, Dept Gerontol, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England

[2] Humboldt Univ, Charite, Inst Pathol, D-10098 Berlin, Germany

来源：

CANCER LETTERS | 2002年 / 176卷 / 01期

关键词：

hTERT gene; telomerase; human lung cancer cell;

D O I：

10.1016/S0304-3835(01)00644-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Maintenance of telomeres, most often by telomerase, is a necessary prerequisite for immortality of eukaryotic cells. To better understand the mechanisms of telomerase up-regulation during tumorigenesis, we analysed the gene dosage of hTERT on chromosome 5p15, a region known to be overrepresented in a variety of malignancies, in 20 lung cancer cell lines by Southern blotting, fluorescence in-situ hybridization, and comparative genomic hybridization. We found a significant correlation between hTERT gene dosage, hTERT mRNA expression and telomerase activity. Imbalances of chromosome 5p may exert functionally relevant hTERT gene dosage effects in human lung cancer. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：81 / 91

页数：11

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