JNK signaling plays an important role in the effects of TNF-α and IL-1β on in vitro osteoblastic differentiation of cultured human periosteal-derived cells

被引：58

作者：

Hah, Young-Sool ^{[1
]}

Kang, Hea-Gea ^{[2
]}

Cho, Hee-Young ^{[1
]}

Shin, Sang-Hoon ^{[2
]}

Kim, Uk-Kyu ^{[2
]}

Park, Bong-Wook ^{[3
]}

Lee, Sang-il ^{[4
]}

Rho, Gyu-Jin ^{[5
]}

Kim, Jong-Ryoul ^{[6
]}

Byun, June-Ho ^{[3
]}

机构：

[1] Gyeongsang Natl Univ Hosp, Clin Res Inst, Jinju, South Korea

[2] Pusan Natl Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Pusan, South Korea

[3] Gyeongsang Natl Univ, Sch Med, Biomed Ctr BK21, Dept Oral & Maxillofacial Surg,Inst Hlth Sci, Jinju 660702, South Korea

[4] Gyeongsang Natl Univ, Sch Med, Biomed Ctr BK21, Dept Internal Med,Inst Hlth Sci, Jinju 660702, South Korea

[5] Gyeongsang Natl Univ, Coll Vet Med, OBS Theriogenol & Biotechnol, Jinju 660702, South Korea

[6] Onhospital, Maxillofacial Ctr, Pusan, South Korea

来源：

MOLECULAR BIOLOGY REPORTS | 2013年 / 40卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

Periosteal-derived cells; TNF-alpha; IL-1; beta; JNK signaling; PROTEIN-KINASE PATHWAY; MESENCHYMAL STEM-CELLS; P38 MAP KINASE; TRANSCRIPTION FACTOR; GENE-EXPRESSION; OSTEOGENIC DIFFERENTIATION; PROINFLAMMATORY CYTOKINES; BONE-FORMATION; ACTIVATION; RUNX2;

D O I：

10.1007/s11033-013-2586-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The purpose of this study was to examine the effects of TNF-alpha and IL-1 beta on in vitro osteoblastic differentiation of cultured human periosteal-derived cells. To examine the effects of TNF-alpha and IL-1 beta on in vitro osteoblastic differentiation of cultured human periosteal-derived cells, the cells cultured in the osteogenic induction medium were treated with 0.1-10 ng/ml TNF-alpha and 0.01-1 ng/ml IL-1 beta. TNF-alpha and IL-1 beta enhanced the alkaline phosphatase (ALP) activity and alizarin red S staining in cultured human periosteal-derived cells. However, these cytokines did not stimulate the Runt-related transcription factor (Runx) 2 activity and osteocalcin secretion. The ALP activity was decreased in the periosteal-derived cells pretreated with mitogen activated protein kinase (MAPK) inhibitors and then treated with TNF-alpha or IL-1 beta. Among the periosteal-derived cells pretreated with MAPK inhibitors, the ALP activity was markedly decreased in the cells pretreated with SP 600125, the specific inhibitor of C-Jun N-terminal kinase (JNK). The periosteal-derived cells treated with TNF-alpha and IL-1 beta showed an increase in extracellular signal-regulated kinase (ERK) and JNK phosphorylation. Among the ERK and JNK phosphorylation, JNK phosphorylation was strongly observed in the cells. These results suggest that TNF-alpha and IL-1 beta increased the in vitro osteoblastic differentiation of cultured human periosteal-derived cells by enhancing the ALP activity and mineralization process, but not by Runx2 activation. The functional role of TNF-alpha and IL-1 beta in increasing the ALP activity and mineralization of periosteal-derived cells primarily depends on the JNK signaling among the MAPK pathways.

引用

页码：4869 / 4881

页数：13

共 33 条

[1]

Tumor Necrosis Factor α and Interleukin-1β Modulate Calcium and Nitric Oxide Signaling in Mechanically Stimulated Osteocytes [J].